Overexpression of SmLEA enhances salt and drought tolerance in Escherichia coli and Salvia miltiorrhiza
Overexpression of SmLEA enhances salt and drought tolerance in Escherichia coli and Salvia miltiorrhiza
复制标题
DOI：
10.1007/s00709-014-0626-z
复制
发表时间：
2014-03
期刊：
影响因子：
2.9
通讯作者：
Yucui Wu;Congling Liu;Jingge Kuang;Q. Ge;Yuan Zhang;Zhe-zhi Wang
中科院分区：
文献类型：
--
作者：
Yucui Wu;Congling Liu;Jingge Kuang;Q. Ge;Yuan Zhang;Zhe-zhi Wang
盐和干旱是限制植物生长发育的重要非生物胁迫。晚期胚胎发育丰富蛋白（莱亚）是一类与植物对水分胁迫的耐受性相关的蛋白质。我们以前从丹参中克隆了一个莱亚基因Sm莱亚。系统发育分析表明，SmLEA属于LEA14组，参与脱水反应。为了详细地确定其功能，我们现在已经在大肠杆菌和S.丹参。SmLEA蛋白在E.大肠杆菌在盐度下比在标准条件下发生得早。当在补充有60 mM NaCl或150 mM甘露醇的MS培养基上培养时，与空载体对照植物相比，millionrhizaplants也显示出更快的根伸长和更低的丙二醛浓度。此外，SmLEA过度表达转基因经历了一个快速失水率。无论是在盐或干旱，过表达的植物有更大的超氧化物歧化酶活性和更高的谷胱甘肽浓度。这些结果表明，SmLEA可能是有用的努力，以提高干旱和耐盐丹参。为进一步研究该药用植物的抗逆机制和分子育种奠定了基础。
Salinity and drought are important abiotic stresses limiting plant growth and development. Late embryogenesis abundant (LEA) proteins are a group of proteins associated with tolerance to water-related stress. We previously cloned an LEA gene,SmLEA, fromSalvia miltiorrhizaBunge. Phylogenetic analysis indicated that SmLEA belongs to Group LEA14, which is involved in the dehydration response. To determine its function in detail, we have now overexpressedSmLEAinEscherichia coliandS. miltiorrhiza. The logarithmic increase in accumulations of SmLEA proteins inE. colioccurred earlier under salinity than under standard conditions.SmLEA-transformedS. miltiorrhizaplants also showed faster root elongation and a lower malondialdehyde concentration than the empty vector control plants did when cultured on MS media supplemented with 60 mM NaCl or 150 mM mannitol. Moreover,SmLEA-overexpressing transgenics experienced a less rapid rate of water loss. Under either salinity or drought, overexpressing plants had greater superoxide dismutase activity and a higher glutathione concentration. These results suggest that SmLEA may be useful in efforts to improve drought and salinity tolerance inS. miltiorrhiza. Our data also provide a good foundation for further studies into the stress resistance mechanism and molecular breeding of this valuable medicinal plant.