Non-climacteric ripening in strawberry fruit is linked to ABA, FaNCED2 and FaCYP707A1
Non-climacteric ripening in strawberry fruit is linked to ABA, FaNCED2 and FaCYP707A1
复制标题
DOI：
10.1071/fp11293
复制
发表时间：
2012-01-01
影响因子：
3
通讯作者：
Leng, Ping
中科院分区：
文献类型：
--
作者：
Ji, Kai;Chen, Pei;Leng, Ping
为研究阿坝在草莓果实发育和成熟中的作用，从草莓品种'Ablion'中克隆了2个阿坝关键合成酶基因FaNCED 1、FaNCED 2和1个阿坝关键降解酶基因FaCYP 707 A1。这三个基因及其推测的氨基酸序列与其它植物中的同源基因有很高的相似性。在草莓果肉中，FaNCED2的表达在两个阶段上升，这与阿坝水平的增加相对应。FaNCED1的表达较低，并持续增加。FaCYP 707 A1的表达量随着果实由亮绿色向白色的转变而增加，随着果实成熟而降低。生长素降低了这三个基因的表达。这些基因在不同器官和组织中的表达分析表明，FaNCED2在成熟瘦果中含量丰富，并且果肉（花托）中有FaNCED1和FaCYP 707 A1的高表达。阿坝可能对草莓果实的发育和成熟起调节作用。阿坝含量受其关键合成基因NCED2和降解基因CYP707A1的调控。
To study the role of ABA in development and ripening of strawberry fruit, two ABA key synthetase genes FaNCED1, FaNCED2 and one ABA key degradation enzyme gene FaCYP707A1 were cloned from strawberry cultivar 'Ablion'. The three genes and putative amino acid sequences, respectively, had high similarities with their homologues in other plants. In strawberry pulp, expression of FaNCED2 rose in two stages that corresponded with increases in ABA levels. The expression of FaNCED1 was much lower and increased continually. The expression of FaCYP707A1 increased as fruit changed from bright green to white, then decreased as it ripened. Auxin reduced expression of these three genes. Analysis of expression of these genes in different organs and tissues showed that FaNCED2 was abundant in mature achenes and the pulp (receptacle) had high expression of FaNCED1 and FaCYP707A1. ABA may play a regulation role in strawberry fruit development and ripening. The content of ABA was regulated by its key synthetase gene NCED2 and degradation gene CYP707A1.