Comparative analysis of metabolic proteome variation in ascorbate-primed and unprimed wheat seeds during germination under salt stress
Comparative analysis of metabolic proteome variation in ascorbate-primed and unprimed wheat seeds during germination under salt stress
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DOI：
10.1016/j.jprot.2014.04.040
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发表时间：
2014-08-28
影响因子：
3.3
通讯作者：
Lagana, Aldo
中科院分区：
文献类型：
--
作者：
Fercha, Azzedine;Capriotti, Anna Laura;Lagana, Aldo
抗坏血酸灌种提高硬粒小麦耐盐性。为了了解这种启动效应的潜在机制，研究人员对未启动和抗坏血酸启动的小麦种子在盐水和非盐水条件下的萌发过程进行了无凝胶鸟枪蛋白质组学分析。由于种子萌发是胚胎和胚周组织相互作用或串扰的结果，我们分别研究了这两个组织中代谢蛋白质组的变化。在胚胎和胚胎周围组织中，697个鉴定蛋白中的167个和471个鉴定蛋白中的69个，在启动和/或盐度的影响下，丰度分别显著增加或减少。在未处理的小麦胚中，盐胁迫导致129种蛋白质发生变化，其中大部分属于代谢、能量、疾病/防御、蛋白质目的地和储存类。抗坏血酸预处理可以防止和抵消盐度对这些蛋白质的影响，并改变其他35种蛋白质的丰度，其中大多数涉及代谢、蛋白质目的地和储存类别。分层聚类分析显示，胚胎和胚胎周围组织中分别存在3个和2个主要的蛋白表达簇。本研究为了解植物诱导的耐盐性开辟了新的途径。为了清楚地了解抗坏血酸是如何增强硬粒小麦在萌发过程中的耐盐性的，我们首次对未启动和抗坏血酸启动的小麦种子在盐水和非盐水条件下的萌发过程进行了比较的散弹枪蛋白质组学分析。此外，由于种子萌发是胚胎和胚周组织相互作用或串扰的结果，我们分别分析了两种组织中代谢蛋白质组的变化。共鉴定出1168种分子量差异较大的蛋白（5 ~ 258 kDa）。其中，167种和69种蛋白在胚胎组织和胚周组织中丰度分别显著高于或低于对照。抗坏血酸预处理减轻了盐度对大多数这些蛋白质的影响，特别是那些参与代谢、能量、疾病/防御、蛋白质目的地和储存功能的蛋白质。分层聚类分析显示，胚胎和胚胎周围组织中分别存在3个和2个主要的蛋白质聚集簇。这些结果可能为了解和推进作物的引物诱导耐盐性提供新的途径。(C) 2014 Elsevier B.V.版权所有
Seed priming with ascorbic acid improves salt tolerance in durum wheat. For understanding the potential mechanisms underlying this priming effect a gel-free shotgun proteomic analysis was performed comparing unprimed to ascorbate-primed wheat seed during germination under saline and non-saline conditions. Since seed germination is the result of interplay or cross-talk between embryo and embryo-surrounding tissues, we studied the variation of metabolic proteome in both tissues separately. 167 of 697 identified and 69 of 471 identified proteins increase or decrease in abundance significantly in response to priming and/or salinity compared to untreated, unstressed control in embryo and embryo-surrounding tissues, respectively. In untreated wheat embryo salt stress was accompanied by change in 129 proteins, most of which are belonging to metabolism, energy, disease/defense, protein destination and storage categories. Ascorbate pretreatment prevents and counteracts the effects of salinity upon most of these proteins and changes specifically the abundance of 35 others proteins, most of which are involved in metabolism, protein destination and storage categories. Hierarchical clustering analysis revealed three and two major clusters of protein expression in embryo and embryo-surrounding tissues, respectively. This study opens promising new avenues to understand priming-induced salt tolerance in plants.Biological significanceTo clearly understand how ascorbate-priming enhance the salt tolerance of durum wheat during germination, we performed for the first time a comparative shotgun proteomic analysis between unprimed and ascorbate-primed wheat seeds during germination under saline and non-saline conditions. Furthermore, since seed germination is the result of interplay or cross-talk between embryo and embryo-surrounding tissues we analyzed the variation of metabolic proteome in both tissues separately. 1168 proteins exhibiting greater molecular weight diversity (ranging from 5 to 258 kDa) were identified. Among them, 167 and 69 proteins were increased or decreased in abundance significantly by priming and/or salinity as compared to control, in embryo and embryo-surrounding tissues respectively. Ascorbate pretreatment alleviates the effects of salinity upon most of these proteins, particularly those involved in metabolism, energy, disease/defense, protein destination and storage functions. Hierarchical clustering analysis revealed three and two major clusters of protein accumulation in embryo and embryo-surrounding tissues, respectively. These results may provide new avenues for understanding and advancing priming-induced salt tolerance in crop plants. (C) 2014 Elsevier B.V. All rights reserved.