Origin of periodic acid-Schiff-reactive glycoprotein in human eccrine sweat.
Origin of periodic acid-Schiff-reactive glycoprotein in human eccrine sweat.
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人类小汗腺汗液中高碘酸希夫反应性糖蛋白的起源。
DOI：
10.1152/jappl.1986.60.5.1615
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发表时间：
1986
影响因子：
3.3
通讯作者：
K. Sato
中科院分区：
文献类型：
--
作者：
S. Yanagawa;H. Yokozeki;K. Sato
为了评估导管阻塞与高碘酸希夫（PAS）阳性物质在人体内的汗腺减数分裂机制中的可能参与，将皮肤切片与乙酰甲胆碱孵育2小时，PAS阳性物质在组织学上定位在导管腔中。在20%的腺体中，观察到PAS阳性物质完全阻塞导管。这种PAS阳性糖蛋白在人体汗液中的特点和起源，然后使用各种电泳技术进行了研究。一维十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（1-D SDS-PAGE）显示相当大的个体差异的电泳模式;然而，四个主要的条带在45，28，20，和18 K共享不同的个人，PAS阳性。使用二维SDS-PAGE、免疫扩散和免疫亲和色谱法的进一步研究表明，PAS阳性糖蛋白不是直接来源于血清，因为它们在免疫学和抗原性上不同于血清蛋白，包括α 1-糖蛋白、α 2-HS-糖蛋白和α 1-抗胰蛋白酶。由于在PAS组织化学染色中仅暗细胞颗粒被密集染色，并且由于针对PAS阳性条带产生的抗血清选择性地染色面向分泌螺旋腔的细胞（其最可能是暗细胞），因此建议PAS阳性汗液糖蛋白主要来自暗细胞。
To evaluate the possible involvement of ductal blockade with periodic acid-Schiff (PAS)-positive materials in the mechanism of hidromeiosis in humans, skin slices were incubated with methacholine for 2 h and PAS-positive materials localized histologically in the ductal lumen. In 20% of the glands complete ductal blockade with PAS-positive materials was noted. The characteristics and origin of such PAS-positive glycoproteins in human sweat were then studied using various electrophoretic techniques. One-dimensional sodium dodecylsulfate-polyacrylamide gel electrophoresis (1-D SDS-PAGE) demonstrated considerable individual variation in the electrophoretic pattern; however, four major bands at 45, 28, 20, and 18K shared by different individuals, were PAS positive. Further studies using two-dimensional SDS-PAGE, immunodiffusion and immunoaffinity chromatography demonstrated that the PAS-positive glycoproteins are not derived directly from serum because they are electrophoretically and antigenically distinct from serum proteins, including alpha 1-glycoprotein, alpha 2-HS-glycoprotein, and alpha 1-antitrypsin. Since only dark cell granules are densely stained in the histochemical PAS staining, and because antiserum produced against the PAS-positive band selectively stained cells facing the secretory coil lumen (which are most likely dark cells), it is suggested that PAS-positive sweat glycoproteins are derived predominantly from the dark cells.