PURIFICATION OF A YEAST PROTEIN THAT BINDS TO ORIGINS OF DNA-REPLICATION AND A TRANSCRIPTIONAL SILENCER
PURIFICATION OF A YEAST PROTEIN THAT BINDS TO ORIGINS OF DNA-REPLICATION AND A TRANSCRIPTIONAL SILENCER
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DOI：
10.1073/pnas.85.7.2120
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发表时间：
1988-04-01
影响因子：
11.1
通讯作者：
STILLMAN, B
中科院分区：
文献类型：
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作者：
DIFFLEY, JFX;STILLMAN, B
在全细胞酵母提取物中鉴定了两个与染色体复制子（自主复制序列1（ARS 1））特异性相互作用的因子。这些蛋白质之一，ARS结合因子I（ABF-I），被纯化为135 kDa的多肽的同质性。当酵母在某些营养条件下生长时，ABF-I结合在先前显示对ARS 1的功能是必需的区域内，并且这里提出的缺失分析表明，含有ABF-I结合位点的ARS 1结构域（结构域B）是由至少两个亚结构域组成的复杂元件。ABF-I还结合在HMR沉默交配基因座的ARS内。该ARS作为顺式作用的转录沉默物起作用，并且该ARS内的ABF-I结合位点对于ARS和沉默物功能都是重要的。
Two factors that interact specifically with the chromosomal replicator, autonomously replicating sequence 1 (ARS1), were identified in whole-cell yeast extracts. One of these proteins, ARS binding factor I (ABF-I), was purified to homogeneity as a polypeptide of 135 kDa. ABF-I binds within a region previously shown to be essential for the function of ARS1 when yeast are grown under certain nutritional conditions, and deletion analysis presented here suggests that the ARS1 domain containing the ABF-I-binding site, domain B, is a complex element composed of at least two subdomains. ABF-I also binds within an ARS at the HMR silent mating locus. This ARS functions as a cis-acting transcriptional silencer, and the ABF-I-binding site within this ARS is important for both ARS and silencer function.