Histochemical Characterization of the Dorsal Raphe-Periaqueductal Grey Dopamine Transporter Neurons Projecting to the Extended Amygdala
Histochemical Characterization of the Dorsal Raphe-Periaqueductal Grey Dopamine Transporter Neurons Projecting to the Extended Amygdala
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DOI：
10.1523/eneuro.0121-22.2022
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发表时间：
2022-05
期刊：
影响因子：
3.4
通讯作者：
Qin Zhao;Tetsufumi Ito;Chika Soko;Yoshie Hori;Takafumi Furuyama;H. Hioki;Kohtarou Konno;Miwako Yamasaki;Masahiko Watanabe;Satoshi Ohtsuka;Munenori Ono;N. Kato;R. Yamamoto
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作者：
Qin Zhao;Tetsufumi Ito;Chika Soko;Yoshie Hori;Takafumi Furuyama;H. Hioki;Kohtarou Konno;Miwako Yamasaki;Masahiko Watanabe;Satoshi Ohtsuka;Munenori Ono;N. Kato;R. Yamamoto
摘要中缝背核（DR）含有大量酪氨酸羟化酶（TH）阳性神经元，被认为是多巴胺（DA）能神经元。DR和中脑导水管周围灰质（PAG）区的这些DA神经元（DADR-PAG神经元）是A10簇的一个亚组，已知A10簇是异质的。该DA群投射到杏仁核中央核（CeA）和终纹床核（BNST），并据报道调节各种情感行为。为了表征小鼠DADR-PAG神经元投射至CeA和BNST的组织化学特征，本研究结合荧光金（FG）逆行标记和组织学技术，重点关注TH、多巴胺转运体（DAT）、血管活性肠肽（VIP）和囊泡谷氨酸转运体2（VGlut 2）。为了鉴定推定的DA神经元，使用DAT-Cre：：Ai 14小鼠。结果表明，DATDR-PAG神经元由TH+/VIP-和TH-/VIP+两个亚群组成。DAT+/TH-/VIP+亚群将是非DA非典型DAT神经元。DATDR-PAG神经元与AAV在DAT-Cre小鼠的顺行标记显示，纤维只支配的CeA的外侧部分和卵圆核的BNST。逆行标记FG注射到CeA或BNST显示，这两个亚群同样支配这些地区。此外，使用VGlut 2-Cre：：Ai 14小鼠，结果表明，支配CeA和BNST的TH-/VIP+亚群是VGlut 2阳性神经元。这两个亚群的DATDR-PAG神经元，TH+/VIP-和TH-/VIP+，可能差异干扰扩展杏仁核，从而调节情感行为。
Abstract The dorsal raphe (DR) nucleus contains many tyrosine hydroxylase (TH)-positive neurons which are regarded as dopaminergic (DA) neurons. These DA neurons in the DR and periaqueductal gray (PAG) region (DADR-PAG neurons) are a subgroup of the A10 cluster, which is known to be heterogeneous. This DA population projects to the central nucleus of the amygdala (CeA) and the bed nucleus of the stria terminalis (BNST) and has been reported to modulate various affective behaviors. To characterize, the histochemical features of DADR-PAG neurons projecting to the CeA and BNST in mice, the current study combined retrograde labeling with Fluoro-Gold (FG) and histological techniques, focusing on TH, dopamine transporter (DAT), vasoactive intestinal peptide (VIP), and vesicular glutamate transporter 2 (VGlut2). To identify putative DA neurons, DAT-Cre::Ai14 mice were used. It was observed that DATDR-PAG neurons consisted of the following two subpopulations: TH+/VIP– and TH–/VIP+ neurons. The DAT+/TH–/VIP+ subpopulation would be non-DA noncanonical DAT neurons. Anterograde labeling of DATDR-PAG neurons with AAV in DAT-Cre mice revealed that the fibers exclusively innervated the lateral part of the CeA and the oval nucleus of the BNST. Retrograde labeling with FG injections into the CeA or BNST revealed that the two subpopulations similarly innervated these regions. Furthermore, using VGlut2-Cre::Ai14 mice, it was turned out that the TH–/VIP+ subpopulations innervating both CeA and BNST were VGlut2-positive neurons. These two subpopulations of DATDR-PAG neurons, TH+/VIP– and TH–/VIP+, might differentially interfere with the extended amygdala, thereby modulating affective behaviors.