Shifts in microbial community structure along an ecological gradient of hypersaline soils and sediments
Shifts in microbial community structure along an ecological gradient of hypersaline soils and sediments
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DOI：
10.1038/ismej.2010.3
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发表时间：
2010-06-01
期刊：
影响因子：
11
通讯作者：
Gentry, Terry J.
中科院分区：
文献类型：
--
作者：
Hollister, Emily B.;Engledow, Amanda S.;Gentry, Terry J.
对高盐生态系统的研究通常会产生新的生物，并有助于我们对极端环境的理解。研究人员对位于美国德克萨斯州南部的La Sal del Rey湖的土壤和沉积物进行了调查，以表征其微生物群落的结构和多样性。样本是沿着一个横跨植被覆盖的高地、暴露的湖床沉积物和淹水地点的样带收集的，捕获了广泛的环境和物理和化学梯度。采用群落定量PCR （qPCR）技术，结合标签编码焦磷酸测序、16S rRNA基因克隆和Sanger测序技术，对湖泊土壤和沉积物微生物群落进行了分析。此外，我们还利用多元统计方法确定了土壤环境中序列多样性与异质性之间的共同关系。总体而言，微生物群落的多样性令人惊讶，包含各种分类群，并且与现场含水量，磷和总有机碳浓度以及ph具有显著的相关性。一些个体种群，特别是古细菌，也与钠浓度和电导率盐度相关。在整个样带中，细菌相对于古细菌在数量上占优势，其中变形菌门、拟杆菌门和厚壁菌门占检测到的分类群的大多数。虽然这些分类群的丰度与其他高盐生态系统中描述的相似，但由于测序深度的增加，我们发现了以前在高盐沉积物中没有描述过的分类群。本研究结果为以前未被表征的生态系统提供了新的信息，并显示了高通量测序在复杂生态系统研究中的价值。ISME学报（2010）4,829 -838；doi: 10.1038 / ismej.2010.3;2010年2月4日在线发布
Studies of hypersaline ecosystems often yield novel organisms and contribute to our understanding of extreme environments. Soils and sediments from La Sal del Rey, a previously uncharacterized, hypersaline lake located in southern Texas, USA, were surveyed to characterize the structure and diversity of their microbial communities. Samples were collected along a transect that spanned vegetated uplands, exposed lakebed sediments, and water-logged locations, capturing a wide range of environments and physical and chemical gradients. Community quantitative PCR (qPCR) was used in combination with tag-encoded pyrosequencing, 16S rRNA gene cloning, and Sanger sequencing to characterize the lake's soil and sediment microbial communities. Further, we used multivariate statistics to identify the relationships shared between sequence diversity and heterogeneity in the soil environment. The overall microbial communities were surprisingly diverse, harboring a wide variety of taxa, and sharing significant correlations with site water content, phosphorus and total organic carbon concentrations, and pH. Some individual populations, especially of Archaea, also correlated with sodium concentration and electrical conductivity salinity. Across the transect, Bacteria were numerically dominant relative to Archaea, and among them, three phyla-the Proteobacteria, Bacteroidetes, and Firmicutes-accounted for the majority of taxa detected. Although these taxa were detected with similar abundances to those described in other hypersaline ecosystems, the greater depth of sequencing achieved here resulted in the detection of taxa not described previously in hypersaline sediments. The results of this study provide new information regarding a previously uncharacterized ecosystem and show the value of high-throughput sequencing in the study of complex ecosystems. The ISME Journal (2010) 4, 829-838; doi:10.1038/ismej.2010.3; published online 4 February 2010