A simple and sensitive resonance Rayleigh scattering method for determination of As(III) using aptamer-modified nanogold as a probe.
A simple and sensitive resonance Rayleigh scattering method for determination of As(III) using aptamer-modified nanogold as a probe.
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DOI：
10.1002/bio.2589
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发表时间：
2014-09
期刊：
影响因子：
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通讯作者：
Meiling Tang;G. Wen;Aihui Liang;Zhiliang Jiang
中科院分区：
文献类型：
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作者：
Meiling Tang;G. Wen;Aihui Liang;Zhiliang Jiang
制备了一种简单且选择性的适配体（ssDNA）修饰的纳米金探针（AussDNA），用于测定含有0.05 mol/L NaCl的HEPES缓冲溶液（pH 8.2）中的痕量As（III）。该方法将 AussDNA-As(III) 的适体反应与纳米金聚集体在 278 nm 处的共振瑞利散射 (RRS) 耦合。当As(III)浓度增加时，278 nm处的RRS强度增加，形成更多的纳米金聚集体和稳定的As(III)-ssDNA复合物。在选定条件下，增加的 RRS 强度 (ΔI) 与 As(III) 浓度在 3.8-230.4 ng/mL 范围内呈线性关系，检测限为 1.9 ng/mL。该RRS方法用于检测水样中的As(III)，具有简单、灵敏、选择性好等优点。
A simple and selective aptamer (ssDNA)-modified nanogold probe (AussDNA) was prepared for the determination of trace As(III) in HEPES buffer solution (pH 8.2) containing 0.05 mol/L NaCl. The method coupled the aptamer reaction of AussDNA-As(III) and the resonance Rayleigh scattering (RRS) of nanogold aggregations at 278 nm. When the As(III) concentration increased, the RRS intensity at 278 nm increased to form more nanogold aggregation and a stable As(III)-ssDNA complex. Under selected conditions, the increased RRS intensity (ΔI) was linear to the concentration of As(III) in the range 3.8-230.4 ng/mL, with a detection limit of 1.9 ng/mL. This RRS method was applied to detect As(III) in water samples, with simplicity, sensitivity and selectivity.