Human EIF5A2 an chromosome 3q25-q27 is a phylogenetically conserved vertebrate variant of eukaryotic translation initiation factor 5A with tissue-specific expression
Human EIF5A2 an chromosome 3q25-q27 is a phylogenetically conserved vertebrate variant of eukaryotic translation initiation factor 5A with tissue-specific expression
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DOI：
10.1006/geno.2000.6418
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发表时间：
2001-01-01
期刊：
影响因子：
4.4
通讯作者：
Johansson, HE
中科院分区：
文献类型：
--
作者：
Jenkins, ZA;Hååg, PG;Johansson, HE
真核细胞翻译起始因子5A(EIF5A)是一种与细胞内多胺稳态密切相关的必需蛋白质，它接受亚精胺衍生的翻译后修饰亚硫氨酸，这是eIF5A的生化活动和细胞增殖所必需的，eIF5A蛋白刺激核糖体多肽转移酶，可能参与核质mRNA的运输。我们对eIF5A的分子遗传学知之甚少，本文报道了eIF5A的一个新的系统发育保守基因--人EIF5A2的序列和分子特征。它位于3q25-q27，通常以癌症中的染色体不稳定而闻名。与普遍表达的EIF5A1基因不同，EIF5A2在睾丸和结直肠腺癌中高表达，在脑中中等水平表达。两个EIF5A2 mRNAs共享一个129个核苷酸的5‘非编码区和153个氨基酸的eIF5AII蛋白的编码序列，但拥有两个交替的46和890个核苷酸的3’非编码区，这两个3‘非编码区是通过差异多聚腺苷作用产生的。该蛋白与eIF5AT基因有84%的同源性和94%的相似性，这两个EIF5A基因在脊椎动物中都是保守的。我们的发现进一步支持了多胺代谢蛋白和调节剂的专门基因表达计划，该计划的功能是在精子发生期间维持多胺稳态在较高水平，(C)2001学术出版社。
Eukaryotic translation initiation factor 5A (eIF5A) is an essential protein tightly linked to cellular polyamine homeostasis, It receives the unique spermidine-derived posttranslational modification hypusine that is necessary for eIF5A's biochemical activity and cellular proliferation, The eIF5A protein stimulates ribosomal peptidyl-transferase and may be involved in nucleocytoplasmic mRNA transport. Little is known about the molecular genetics of eIF5A, Here we report on the sequence and molecular characterization of human EIF5A2, a novel phylogenetically conserved gene for eIF5A, EIF5A2 stretches over 17 kb and consists of five exons and four introns. It is localized at 3q25-q27, often noted for chromosomal instability in cancers. EIF5A2 is highly expressed in testis and colorectal adenocarcinoma and at moderate levels in the brain, in contrast to the ubiquitously expressed EIF5A1 gene. Two EIF5A2 mRNAs share a 129-nt 5' UTR and a coding sequence for the 153-amino-acid eIF5AII protein, but possess two alternative 3' UTRs of 46 and 890 nt that arise through differential polyadenylation. The protein is 84% identical and 94% similar to eIF5AT, Both EIF5A genes are conserved in vertebrates. Our findings lend further support for a specialized gene expression program of polyamine metabolic proteins and regulators that function to maintain polyamine homeostasis at elevated levels during spermatogenesis, (C) 2001 Academic Press.