Gene expression profiling of peripheral blood mononuclear cells from cattle infected with Mycobacterium paratuberculosis
Gene expression profiling of peripheral blood mononuclear cells from cattle infected with Mycobacterium paratuberculosis
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DOI：
10.1128/iai.70.10.5494-5502.2002
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发表时间：
2002-10-01
影响因子：
3.1
通讯作者：
Sipkovsky, S
中科院分区：
文献类型：
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作者：
Coussens, PM;Colvin, CJ;Sipkovsky, S
利用包含721个独特的白细胞表达序列标签(EST)和代表已知基因的扩增片段的牛特异性基因芯片系统，比较了临床和亚临床约翰氏病阳性荷斯坦奶牛(每组2头)外周血单个核细胞(PBMC)的基因表达谱。刺激临床感染副结核分枝杆菌的奶牛的PBMC往往会降低83个基因的表达(倍数变化，>；1.5)。在这83个基因中，有16个基因在两个临床奶牛中均有显著下调(P1.5；P1.5；P
A bovine-specific cDNA microarray system containing 721 unique leukocyte expressed sequence tags (ESTs) and amplicons representing known genes was used to compare gene expression profiles of peripheral blood mononuclear cells (PBMCs) from clinical and subclinical Johne's disease-positive Holstein cows (n=2 per group). Stimulation of PBMCs from clinically infected cows with Mycobacterium paratuberculosis tended to decrease expression of 83 genes (fold change, >1.5). Of these 83 genes, 16 displayed significant down regulation across both clinical cows (P1.5; P1.5; P