Fructanolytic and Saccharolytic Enzymes of the Rumen Bacterium Pseudobutyrivibrio ruminis strain 3-preliminary study
Fructanolytic and Saccharolytic Enzymes of the Rumen Bacterium Pseudobutyrivibrio ruminis strain 3-preliminary study
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DOI：
10.1007/s12223-010-0051-4
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发表时间：
2010-07-01
影响因子：
2.6
通讯作者：
Michalowski, T.
中科院分区：
文献类型：
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作者：
Kasperowicz, A.;Stan-Glasek, K.;Michalowski, T.
从绵羊瘤胃中分离到一株瘤胃普立克次体3号菌株，并对其16 S rRNA基因进行了序列分析。该菌能以牧草果聚糖、菊粉、蔗糖、果糖和葡萄糖为唯一碳源生长，群体吸光度在0.4- 1.2之间。在1天内，细菌消耗了初始剂量的92- 97%的菊粉（其利用率不超过33%）。该细菌细胞提取物催化与生长培养基中存在的碳源相关的梯牧草果聚糖、菊粉和蔗糖的降解。采用SephadexG- 150分子筛过滤、聚丙烯酰胺凝胶电泳、酶谱分析和薄层层析等技术对蔗糖和果糖两种聚合物的酶系进行了鉴定。两种特异性内切酶（EC 3.2.1.65），非特异性。- 在生长在蒂莫西草果聚糖上的细菌的无细胞提取物中检测到呋喃果糖苷酶（3.2.1.80和/或3.2.1.26）和蔗糖磷酸化酶（2.4.1.7）。
P. ruminis strain 3 was isolated from the ovine rumen and identified on the basis of comparison of its 16S rRNA gene with GenBank. The bacterium was able to grow on Timothy grass fructan, inulin, sucrose, fructose and glucose as a sole carbon source, reaching absorbance of population in a range of 0.4- 1.2. During 1 d the bacteria exhausted 92- 97 % of initial dose of saccharides except for inulin (its utilization did not exceed 33 %). The bacterial cell extract catalyzed the degradation of Timothy grass fructan, inulin and sucrose in relation to carbon source present in growth medium. Molecular filtration on Sephadex G- 150, polyacrylamide gel electrophoresis combined with zymography technique and TLC was used to identify enzymes responsible for the digestion of sucrose and both polymers of fructose. Two specific endolevanases (EC 3.2.1.65), nonspecific. - fructofuranosidase (EC 3.2.1.80 and/ or EC 3.2.1.26) and sucrose phosphorylase (EC 2.4.1.7) were detected in cell- free extract from bacteria grown on Timothy grass fructan.