Assessment of regional myocardial and renal blood flow with copper-PTSM and positron emission tomography.
Assessment of regional myocardial and renal blood flow with copper-PTSM and positron emission tomography.
复制标题
使用铜-PTSM 和正电子发射断层扫描评估局部心肌和肾血流量。
DOI：
10.1161/01.cir.82.3.990
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发表时间：
1990
期刊：
影响因子：
37.8
通讯作者：
Bergmann,SR
中科院分区：
文献类型：
--
作者：
Shelton,ME;Green,MA;Mathias,CJ;Welch,MJ;Bergmann,SR
我们最近在离体灌注心脏中证明，放射性标记的异戊醛双（N4-甲基氨基硫脲）铜（II）（Cu-PTSM）在包括缺血、缺氧和充血在内的一系列条件下均能很好地提取。一旦被提取，离体心脏与放射性的结合基本上是不可逆的，从而使这种示踪剂具有微球样的性质。由于铜-PTSM可以很容易地制备与发电机产生的正电子发射铜62和其他γ-或正电子发射铜放射性核素，我们评估了它的有用性，测量局部心肌和肾血流量在体内完整的狗在休息，缺血后，或后冠状动脉充血诱导静脉注射潘生丁。静脉注射放射性标记的Cu-PTSM后，示踪剂迅速从血液中清除。在示踪剂给药后15分钟，在心肌样品中直接测量了单光子发射67 Cu标记的Cu-PTSM的心肌摄取，并且在整个流量范围内，其与血流成比例地增加（估计伴随放射性标记微球）为0.0-6.0 ml/g/min（n = 17只狗的340个样品，r = 0.99，Y铜放射性= 85 X微球流量-7 χ 2 + 17）。放射性标记的Cu-PTSM的肾摄取也与血流量成正比。静脉注射64 Cu标记的Cu-PTSM（19%正电子衰变，t1/2 = 12.8小时）后，在4只完整犬中进行正电子发射断层扫描。获得了心脏和肾脏的高质量图像。因此，放射性标记的铜PTSM应该是一个有用的，发电机产生的示踪剂，估计区域心肌和肾血流量与正电子发射断层扫描。
We recently demonstrated in isolated, perfused hearts that radiolabeled pyruvaldehyde bis(N4-methylthiosemicarbazonato)copper(II) (Cu-PTSM) is well extracted throughout a range of conditions including ischemia, hypoxia, and hyperemia. Once extracted, binding of radioactivity by the isolated heart was essentially irreversible, giving this tracer microspherelike qualities. Because Cu-PTSM can be readily prepared with the generator-produced positron-emitting copper 62 and other gamma- or positron-emitting copper radionuclides, we evaluated its usefulness for measuring regional myocardial and renal blood flow in vivo in intact dogs at rest, after ischemia, or after coronary hyperemia was induced by intravenous administration of dipyridamole. After intravenous administration of radiolabeled Cu-PTSM, the tracer cleared rapidly from the blood. Myocardial uptake of single photon-emitting 67Cu-labeled Cu-PTSM was measured directly in myocardial samples 15 minutes after tracer administration, and it increased proportionally with blood flow throughout the flow range (estimated concomitantly with radiolabeled microspheres) of 0.0-6.0 ml/g/min (n = 340 samples from 17 dogs, r = 0.99, Ycopper radioactivity = 85Xmicrosphere flow -7 chi 2 + 17). Renal uptake of radiolabeled Cu-PTSM was also proportional to blood flow. Positron emission tomography was performed in four intact dogs after intravenous administration of 64Cu-labeled Cu-PTSM (19% positron decay, t1/2 = 12.8 hours). High-quality images of heart and kidney were obtained. Accordingly, radiolabeled Cu-PTSM should be a useful, generator-produced tracer for estimating regional myocardial and renal blood flow with positron emission tomography.