A HIGH-PRESSURE LIQUID-CHROMATOGRAPHIC METHOD FOR PLASMA PHENYLACETIC ACID, A PUTATIVE MARKER FOR DEPRESSIVE-DISORDERS
A HIGH-PRESSURE LIQUID-CHROMATOGRAPHIC METHOD FOR PLASMA PHENYLACETIC ACID, A PUTATIVE MARKER FOR DEPRESSIVE-DISORDERS
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DOI：
10.1016/0003-2697(85)90333-1
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发表时间：
1985-01-01
影响因子：
2.9
通讯作者：
SABELLI, H
中科院分区：
文献类型：
--
作者：
GUSOVSKY, F;FAWCETT, J;SABELLI, H
描述了测定人血浆中总苯乙酸（PAA）的HPLC [高压液相色谱]方法。用0.4N HClO 4沉淀血浆蛋白后，用1.5N HCl在100 ℃水解上清液。PAA用苯萃取。将PAA从有机层反萃取到0.5ml 0.1N NaOH中。用HCl中和后，将样品直接进样至HPLC柱（C18）上。采用紫外检测器在210 nm处检测PAA。对照群体的血浆PAA值（536.18 ±. 54.99ng/ml，N = 10）（X . ±. SE）与使用GC/MS [气相色谱-质谱]方法报告的值一致。抑郁的受试者显示出显著较低的值（327.64 ±. 45.44 ng/ml，N = 10），支持PAA可能是抑郁症标志物的观点。
An HPLC [high pressure liquid chromatography] procedure for the determination of total phenylacetic acid (PAA) in human plasma is described. After precipitation of plasma proteins with 0.4 N HClO4, the supernatant was hydrolyzed with 1.5 N HCl at 100.degree. C for 5 h; PAA was extracted with benzene. From the organic layer PAA was back-extracted into 0.5 ml of 0.1 N NaOH. After neutralization with HCl the sample was directly injected onto the HPLC column (C18). An ultraviolet detector at 210 nm was used to monitor PAA. The plasma PAA values for a control population (536.18 .+-. 54.99 ng/ml, N = 10) (X .+-. SE) obtained by the described method are in agreement with values reported using GC/MS [gas chromatography-mass spectrometry] methods. Depressed subjects showed significantly lower values (327.64 .+-. 45.44 ng/ml, N = 10), supporting the view that PAA may be a marker for depressive disorders.