Validation of an in vitro biofilm model of supragingival plaque
Validation of an in vitro biofilm model of supragingival plaque
复制标题
DOI：
10.1177/00220345010800011201
复制
发表时间：
2001-01-01
影响因子：
7.6
通讯作者：
Shapiro, S
中科院分区：
文献类型：
--
作者：
Guggenheim, B;Giertsen, E;Shapiro, S
生物膜结构和功能的研究要求使用可以严格控制许多环境变量的模型系统。我们描述了包含内斯伦迪放线菌、迪斯帕韦荣球菌、具核梭杆菌、远亲链球菌和口腔链球菌的龈上菌斑模型，其中细胞在涂有唾液膜的羟基磷灰石盘上的基于唾液的培养基中厌氧培养，使用所有人通用的材料和装置 微生物学实验室。 0.5小时、16.5小时、40.5小时和64.5小时后，通过培养技术、活/死荧光染色和共焦激光扫描显微镜分析粘附生物膜的组成。重复的独立试验证明了 40.5 小时和 64.5 小时后生物膜形成的可重复性。生物膜短暂暴露于氯己定或三氯生会导致与体内观察到的类似的活力损失。该生物膜模型对于临床相关浓度的预期抗牙菌斑药物的临床前测试非常有用。
The study of biofilm structure and function mandates the use of model systems for which a host of environmental variables can be rigorously controlled. We describe a model of supragingival plaque containing Actinomyces naeslundii, Veillonella dispar, Fusobacterium nucleatum, Streptococcus sobrinus, and Streptococcus oralis wherein cells are cultivated anaerobically in a saliva-based medium on hydroxyapatite discs coated with a salivary pellicle, with material and pieces of apparatus common to all microbiology laboratories. After 0.5 hr, 16.5 hrs, 40.5 hrs, and 64.5 hrs, the composition of adherent biofilms was analyzed by culture techniques, live/dead fluorescence staining, and confocal laser scanning microscopy. Repeated independent trials demonstrated the repeatability of biofilm formation after 40.5 hrs and 64.5 hrs. Brief exposures of biofilms to chlorhexidine or Triclosan produced losses in viability similar to those observed in vivo. This biofilm model should prove very useful for pre-clinical testing of prospective anti-plaque agents at clinically relevant concentrations.