Phylogenetic analysis of multiple heterosexual transmission events involving subtype B of HIV type 1
Phylogenetic analysis of multiple heterosexual transmission events involving subtype B of HIV type 1
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DOI：
10.1089/088922201750236960
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发表时间：
2001-05-20
影响因子：
1.5
通讯作者：
Balfe, P
中科院分区：
文献类型：
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作者：
Hayman, A;Moss, T;Balfe, P
1996年至1999年，在英格兰北部的一个小镇唐卡斯特（Doncaster）（人口约25万）发现了13例艾滋病毒感染病例，涉及当地夜总会的共享性历史的复杂网络将这些病例联系起来，唯一已知的风险因素是异性性交。在1998-1999年收集了一系列冷冻血液样品，并通过PCR扩增以产生全长gp 120克隆。测序结果表明，在这个异性恋群体中的所有传播事件都涉及HIV-1的B亚型，当评估样本之间的关系时，很明显，这13例病例至少代表了三种不同的HIV-1毒株，表明HIV在这个社区已经很好地建立起来。13例中有11例是相关的，形成了两个不同的组。进一步的调查显示，第一组包含5名患者，他们的总体健康状况良好，没有接受HAART治疗。相反，第二组6名患者，包括假定的索引病例，有症状，接受HAART治疗，可能感染了CXCR-4利用病毒。有几个通过遗传标准联系起来的病例没有通过接触追踪联系起来，这意味着在这个社区可能存在更多未确诊的病例，据我们所知，这是迄今为止在联合王国异性恋社区内研究的最大规模的艾滋病毒爆发，表明这种感染途径在联合王国变得越来越普遍。
Between 1996 and 1999 thirteen cases of HIV infection were detected in Doncaster, a small town in the north of England (population similar to 250,000), A complex network of shared sexual histories involving local nightclubs linked these cases, with the only known risk factor being heterosexual intercourse. A series of frozen blood samples was collected in 1998-1999 and amplified by PCR to generate full-length gp120 clones. Sequencing demonstrated that all the transmission events in this heterosexual group involved the B subtype of HIV-1, When relationships between the samples were assessed it became clear that these 13 cases represented at least three separate strains of HIV-1, indicating that HIV is well established in this community. Eleven of the 13 cases were related, forming two distinct groups. Further investigation revealed that one group contained five patients whose general health was good and who were not receiving HAART, In contrast, the second group of six patients, including the putative index case, were symptomatic, receiving HAART, and may have been infected with a CXCR-4-utilizing virus. Several of the cases that were linked by genetic criteria were not linked by contact tracing, implying that further undiagnosed cases may exist in this community, To our knowledge, this is the largest outbreak of HIV studied within the heterosexual community in the United Kingdom to date, suggesting that this route of infection is becoming more common within the United Kingdom.