Expression of HOX gene products in normal and abnormal trophoblastic tissue
Expression of HOX gene products in normal and abnormal trophoblastic tissue
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DOI：
10.1016/s0090-8258(03)00357-3
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发表时间：
2003-09-01
影响因子：
4.7
通讯作者：
Pfaff-Amesse, T
中科院分区：
文献类型：
--
作者：
Amesse, LS;Moulton, R;Pfaff-Amesse, T
Objective.在正常人胎盘组织和来自完全性葡萄胎和绒毛膜癌肿瘤的异常滋养层组织中检测了三种同源异型盒基因产物HOX A1、HOX B6和HOX C6的表达模式。我们试图确定这些基因产物在滋养层细胞分化和增殖的不同状态下的表达是恒定的还是表现出变化。这些同源异型盒基因表达的变化可能为预测哪种磨牙组织可能发展为绒毛膜癌提供依据。研究了来自总共12个样品的组织切片。其中，6例足月人胎盘、3例完全性葡萄胎和3例绒毛膜癌组织采用免疫组化染色方法检测同源框HOX A 11、HOX B6和HOX C6基因产物的表达。HOX同源盒基因产物HOX A11、HOX B6和HOX C6在足月人胎盘和完全性葡萄胎组织中的表达被检测到。从完整的痣异常滋养细胞表现出的免疫反应性表达模式与正常滋养细胞从长期妊娠。然而，在绒癌肿瘤组织中并未发现这些相应的同源盒基因的明确表达。结论。HOX同源框基因产物HOX A11、HOX B6和HOX C6的表达变化在从足月人胎盘、完全性葡萄胎和绒毛膜癌肿瘤中获得的滋养层组织中建立。这一发现表明，正常的足月滋养细胞和异常的磨牙滋养细胞可能有着相似的基本调控机制。这些HOX基因产物在来自绒毛膜癌肿瘤的滋养层细胞中的确定表达的缺乏表明，虽然HOX A11、HOX B6和HOX C6基因可能参与维持某些滋养层细胞状态，但它们在来自绒毛膜癌肿瘤的滋养层细胞中的表达可能下调或改变。(C)2003 Elsevier Science（美国）。All rights reserved.
Objective. The expression pattern of three homeobox genes products, HOX All, HOX B6, and HOX C6, was examined in normal human placental tissue and abnormal trophoblastic tissue derived from complete hydatidiform moles and choriocarcinoma tumors. We sought to determine whether expression of these gene products during different states of trophoblastic differentiation and proliferation is constant or demonstrates variation. Variation in expression of these respective homeobox genes may provide insight into predicting which molar tissues are likely to develop into choriocarcinoma tumors.Methods. Tissue sections from a total of 12 samples were studied. Among these, six full-term human placentas, three complete hydatidiform moles, and three choriocarcinoma tumors were examined for expression of the homeobox HOX A 11, HOX B6, and HOX C6 gene products, using immunohistochemistry staining methods.Results. Expression of HOX homeobox gene products, HOX A11, HOX B6, and HOX C6, was detected in full-term human placenta and tissue from complete hydatiform moles. Abnormal trophoblasts from complete moles demonstrated an immunoreactivity expression pattern comparable to that of normal trophoblasts from term pregnancies. However, definitive expression of these respective homeobox genes was not identified in tissue obtained from choriocarcinoma tumors.Conclusion. Variation in expression of HOX homeobox gene products, HOX A11, HOX B6, and HOX C6, was established in trophoblast tissue obtained from full-term human placentas, complete hydatiform moles, and choriocarcinoma tumors. This finding indicates that normal full-term trophoblasts and abnormal molar trophoblasts may share similar fundamental regulatory control mechanisms. The absence of definitive expression of these HOX gene products in trophoblastic cells derived from choriocarcinoma tumors indicates that while HOX A11, HOX B6, and HOX C6 genes may be involved in maintenance of some trophoblastic cell states, they may be either downregulated or have alterations in their expression in trophoblasts from choriocarcinoma tumors. (C) 2003 Elsevier Science (USA). All rights reserved.