BCL2 and BCL3 are recurrent translocation partners of the IGH locus
BCL2 and BCL3 are recurrent translocation partners of the IGH locus
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DOI：
10.1016/j.cancergencyto.2008.06.007
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发表时间：
2008-10-15
影响因子：
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通讯作者：
Siebert, Reiner
中科院分区：
文献类型：
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作者：
Szymanowska, Natalia;Klapper, Wolfram;Siebert, Reiner
染色体易位影响免疫球蛋白重链（IGH）位点（染色体带14q32）是b细胞淋巴瘤中最常见的细胞遗传学改变。我们利用CD30联合免疫荧光和间期细胞遗传学（FICTION技术）研究了连续确定的94例经典霍奇金淋巴瘤（cHL）中IGH易位的存在。87例可评估病例中有11例（13%）的霍奇金细胞和里德-斯滕伯格细胞显示提示IGH易位的信号模式。为了确定易位伙伴，我们进一步对这些病例进行了MYC、BCL2、BCL6、BCL3、REL/BCLIIA、JAK2/PDCDILG2（别名PDL2） C14orf43和C2TA位点的检测。在4例cHL中发现了IGH易位伴侣，其中BCL2和BCL3各2例。在合适材料的病例中，免疫组化结果显示，IGH/BCL2融合cHL和IGH/BCL3融合cHL的肿瘤细胞分别表达BCL2和BCL3蛋白。这些数据表明，BCL2或BCL3是cHL中IGH位点的反复易位伙伴，然而，cHL中IGH易位的大多数易位伙伴仍有待确定。(C) 2008爱思唯尔公司版权所有。
Chromosomal translocations affecting the immunoglobulin heavy chain (IGH) locus ill chromosomal band 14q32 are the most frequent cytogenitic changes in B-cell lymphomas We studied the presence of IGH translocations in a consecutively ascertained series of 94 classical Hodgkin lymphomas (cHL) by combined immunofluorescence for CD30 and interphase cytogenetics (FICTION technique). The Hodgkin and Reed-Sternberg cells of a total of 11 of 87 evaluable cases (13%) showed signal patterns indicative of IGH translocations. To identity the translocation partners, these cases were further studied with probes for the MYC, BCL2, BCL6, BCL3, REL/BCLIIA, JAK2/PDCDILG2 (alias PDL2) C14orf43, and C2TA loci. The IGH translocation partner could be identified in four cHL and involved BCL2 and BCL3 in two cases each. Immunohistochemistry in cases with suitable material revealed that tumor cells of the two cHL with IGH/BCL2 fusion and the cHL with IGH/BCL3 fusion expressed the BCL2 and BCL3 protein, respectively. These data indicate that BCL2 or BCL3 are recurrent translocation partners of the IGH locus in cHL: however, most of the translocation partners of IGH translocations in cHL remain to be identified. (C) 2008 Elsevier Inc. All rights reserved.