Analysis of genomic DNA of DcACS1, a 1-aminocyclopropane-1-carboxylate synthase gene, expressed in senescing petals of carnation (Dianthus caryophyllus) and its orthologous genes in D. superbus var. longicalycinus
Analysis of genomic DNA of DcACS1, a 1-aminocyclopropane-1-carboxylate synthase gene, expressed in senescing petals of carnation (Dianthus caryophyllus) and its orthologous genes in D. superbus var. longicalycinus
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DOI：
10.1007/s00299-010-0962-1
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发表时间：
2011-04-01
影响因子：
6.2
通讯作者：
Satoh, Shigeru
中科院分区：
文献类型：
--
作者：
Harada, Taro;Murakoshi, Yuino;Satoh, Shigeru
康乃馨（石竹）花表现出更年期乙烯的产生，随后花瓣枯萎，这是一种衰老症状。 DcACS1 编码 1-氨基环丙烷-1-羧酸合酶 (ACS)，是参与这种现象的基因。我们通过基因组 PCR 确定了 DcACS1 的基因组 DNA 结构。在“浅粉红芭芭拉”的基因组中，我们发现了两种不同的核苷酸序列：一种对应于之前显示为 DcACS1 的基因，此处指定为 DcACS1a，另一种新的序列指定为 DcACS1b。结果表明，DcACS1a和DcACS1b都有5个外显子和4个内含子。这两个基因在外显子中具有几乎相同的核苷酸序列，但在某些内含子和3'-UTR中则不同。转录积累分析表明，DcACS1b 在衰老花瓣中与 DcACS1a 一样表达。对32个康乃馨品种的基因组PCR分析表明，大多数品种仅含有DcACS1a，有些品种同时含有DcACS1a和DcACS1b。此外，我们还从 D. superbus var. 中发现了两个具有不同核苷酸序列的 DcACS1 直系同源基因。 longicalycinus，并将它们命名为 DsuACS1a 和 DsuACS1b。 D. superbus var. 的花瓣longicalycinus 响应外源乙烯而产生乙烯，并伴随着 DsuACS1 转录物的积累。这些数据表明，花朵中更年期乙烯的产生是在康乃馨栽培之前从基因上确定的。
Carnation (Dianthus caryophyllus) flowers exhibit climacteric ethylene production followed by petal wilting, a senescence symptom. DcACS1, which encodes 1-aminocyclopropane-1-carboxylate synthase (ACS), is a gene involved in this phenomenon. We determined the genomic DNA structure of DcACS1 by genomic PCR. In the genome of 'Light Pink Barbara', we found two distinct nucleotide sequences: one corresponding to the gene previously shown as DcACS1, designated here as DcACS1a, and the other novel one designated as DcACS1b. It was revealed that both DcACS1a and DcACS1b have five exons and four introns. These two genes had almost identical nucleotide sequences in exons, but not in some introns and 3'-UTR. Analysis of transcript accumulation revealed that DcACS1b is expressed in senescing petals as well as DcACS1a. Genomic PCR analysis of 32 carnation cultivars showed that most cultivars have only DcACS1a and some have both DcACS1a and DcACS1b. Moreover, we found two DcACS1 orthologous genes with different nucleotide sequences from D. superbus var. longicalycinus, and designated them as DsuACS1a and DsuACS1b. Petals of D. superbus var. longicalycinus produced ethylene in response to exogenous ethylene, accompanying accumulation of DsuACS1 transcripts. These data suggest that climacteric ethylene production in flowers was genetically established before the cultivation of carnation.