Differences in miRNA expression profiles between GIST and leiomyoma in human samples acquired by submucosal tunneling biopsy.
Differences in miRNA expression profiles between GIST and leiomyoma in human samples acquired by submucosal tunneling biopsy.
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DOI：
10.1055/s-0034-1393077
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发表时间：
2015-12
影响因子：
2.6
通讯作者：
Masaki T
中科院分区：
文献类型：
--
作者：
Fujita K;Kobara H;Mori H;Fujihara S;Chiyo T;Matsunaga T;Nishiyama N;Ayaki M;Yachida T;Morishita A;Fujiwara M;Okano K;Suzuki Y;Iwama H;Masaki T
背景与研究目的：胃肠道间质小肿瘤（gist）很少有恶性潜能，预后较差。在日常实践中，使用常规影像学来区分具有相似特征的小间质瘤和平滑肌瘤是困难的，但也是必要的。尽管一些研究报道了血清c-kit作为非小GIST和特异性miRNA的生物标志物的效用，但这种测试的临床方面存在争议。本研究的目的是通过研究人类病例中miRNA的表达模式来确定小GIST和平滑肌瘤之间的差异。患者和方法：对9个GIST（小于低风险，平均大小为18 mm）样本和7个平滑肌瘤样本进行了MiRNA表达检测，这些样本是通过一种新的采样方法粘膜下隧道活检（STB）获得的，这种方法产生的粘膜下肿瘤（SMT）的肿瘤标本没有足够大的污染，可以在直接视觉下进行检查。从这些组织中提取总RNA，并通过微阵列分析miRNA的表达模式。随后，使用实时定量聚合酶链反应（qPCR）确认特异性miRNA过表达，并将gist与平滑肌瘤进行比较。结果：微阵列分析显示，与平滑肌瘤相比，gist中miR-140家族的上调高达20倍。Real-time qPCR结果显示，mir -140- 5p在gist中的表达量是平滑肌瘤的27.86倍；mir -140- 3p升高12.24倍。结论：STB法为miRNA分析提供了合适的SMT样本。MiR-140家族成员可以作为区分GIST和平滑肌瘤的特异性生物标志物。
Background and study aims: Small gastrointestinal stromal tumors (GISTs) rarely have malignant potential with poor prognosis. Using conventional imaging to differentiate between small GISTs and leiomyoma, which often have similar characteristics, is difficult but essential in daily practice. Although some studies have reported on the utility of serum c-kit as a biomarker for non-small GIST and specific miRNA, clinical aspects of such testing are controversial. The aim of this study was to identify differences between small GIST and leiomyoma through the investigation of miRNA expression patterns in human cases. Patients and methods: MiRNA expression was examined in nine GIST (less than low risk, mean 18 mm in size) samples and seven leiomyoma samples acquired by a novel sampling method, submucosal tunneling biopsy (STB), which produces tumor specimens of submucosal tumor (SMT) without contamination of sufficient size to be examined under direct vision. Total RNA was extracted from these tissues and analyzed for miRNA expression patterns by microarray. Subsequently, real-time quantitative polymerase chain reaction (qPCR) were used to confirm specific miRNA overexpression, comparing GISTs with leiomyomas. Results: Microarray analysis revealed upregulation of the miR-140 family up to 20 times higher in GISTs than in leiomyomas. Real-time qPCR revealed that the expression level of miR-140-5 p in GISTs was 27.86 times higher than in leiomyomas; miR-140-3 p was 12.24 times higher as well. Conclusions: The STB method provided suitable SMT samples for miRNA analysis. MiR-140 family members may serve as specific biomarkers to distinguish GIST from leiomyoma.