Cross-Talk Between Sporophyte and Gametophyte Generations Is Promoted by CHD3 Chromatin Remodelers in Arabidopsis thaliana
Cross-Talk Between Sporophyte and Gametophyte Generations Is Promoted by CHD3 Chromatin Remodelers in Arabidopsis thaliana
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DOI：
10.1534/genetics.115.180141
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发表时间：
2016-06-01
期刊：
影响因子：
3.3
通讯作者：
Ogas, Joe
中科院分区：
文献类型：
--
作者：
Carter, Benjamin;Henderson, James T.;Ogas, Joe
被子植物的繁殖需要不同基因类型的多个组织的整合发育。为了成功受精，单倍体雌配子体和二倍体子房必须协调它们的发育，之后雄配子必须通过母体孢子体组织到达雌配子。受精后，种子发育需要母体二倍体珠被、三倍体胚乳和二倍体合子的协调发展。转录和信号因子有助于这些组织之间的交流，其中一些过程已经描述了表观遗传调节的作用。在这里，我们确定了CHD3染色质重构体在拟南芥生殖发育中的广泛作用。缺少CHD3重构体的植物泡菜表现出各种生殖缺陷，包括珠被、雌配子体和花粉管的异常发育，以及胚珠和胚胎发育的延迟。遗传分析表明，这些表型是母体孢子体失去泡菜的结果。与泡菜相关的基因2在胚乳中优先表达，并与种子中的泡菜起拮抗作用：失去与泡菜相关的2会抑制泡菜种子的大种子表型。令人惊讶的是，泡菜种子大小的变化足以决定胚性性状在幼苗初级根中的表达。这些发现确立了CHD3重构体在植物繁殖中的重要作用，并强调了一个组织的表观遗传状态如何影响遗传上不同的组织的发育。
Angiosperm reproduction requires the integrated development of multiple tissues with different genotypes. To achieve successful fertilization, the haploid female gametophytes and diploid ovary must coordinate their development, after which the male gametes must navigate through the maternal sporophytic tissues to reach the female gametes. After fertilization, seed development requires coordinated development of the maternal diploid integuments, the triploid endosperm, and the diploid zygote. Transcription and signaling factors contribute to communication between these tissues, and roles for epigenetic regulation have been described for some of these processes. Here we identify a broad role for CHD3 chromatin remodelers in Arabidopsis thaliana reproductive development. Plants lacking the CHD3 remodeler, PICKLE, exhibit various reproductive defects including abnormal development of the integuments, female gametophyte, and pollen tube, as well as delayed progression of ovule and embryo development. Genetic analyses demonstrate that these phenotypes result from loss of PICKLE in the maternal sporophyte. The paralogous gene PICKLE RELATED 2 is preferentially expressed in the endosperm and acts antagonistically with respect to PICKLE in the seed: loss of PICKLE RELATED 2 suppresses the large seed phenotype of pickle seeds. Surprisingly, the alteration of seed size in pickle plants is sufficient to determine the expression of embryonic traits in the seedling primary root. These findings establish an important role for CHD3 remodelers in plant reproduction and highlight how the epigenetic status of one tissue can impact the development of genetically distinct tissues.