ELF3 encodes a circadian clock-regulated nuclear protein that functions in an Arabidopsis PHYB signal transduction pathway
ELF3 encodes a circadian clock-regulated nuclear protein that functions in an Arabidopsis PHYB signal transduction pathway
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DOI：
10.1105/tpc.13.6.1293
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发表时间：
2001-06-01
期刊：
影响因子：
11.6
通讯作者：
Wanger, DR
中科院分区：
文献类型：
--
作者：
Liu, XL;Covington, MF;Wanger, DR
植物发育的许多方面受光感受器功能和生物钟的调节。拟南芥早花3号(ELF3)和光敏色素S(PHY)基因的功能缺失突变导致早花，并影响生物钟调节过程的活性。我们在这里证明了ELF3蛋白的相对丰度，这是一种新的核定位蛋白，表现出遵循ELF3转录本的昼夜积累模式的昼夜调节。此外，在酵母双杂交实验和体外实验中，ELF3蛋白与PHYB相互作用。遗传分析表明，ELF3在早期形态发生中需要PHYB功能，但对开花时间的调控不需要。这表明ELF3是PHYB信号复合体的一个组成部分，它控制植物发育的早期事件，但ELF3和PHYB通过独立的信号转导途径控制开花。
Many aspects of plant development are regulated by photoreceptor function and the circadian clock. Loss-of-function mutations in the Arabidopsis EARLY FLOWERING 3 (ELF3) and PHYTOCHROME S (PHYS) genes cause early flowering and influence the activity of circadian clock-regulated processes. We demonstrate here that the relative abundance of the ELF3 protein, which is a novel nucleus-localized protein, displays circadian regulation that follows the pattern of circadian accumulation of ELF3 transcript. Furthermore, the ELF3 protein interacts with PHYB in the yeast two-hybrid assay and in vitro. Genetic analyses show that ELF3 requires PHYB function in early morphogenesis but not for the regulation of flowering time. This suggests that ELF3 is a component of a PHYB signaling complex that controls early events in plant development but that ELF3 and PHYB control flowering via independent signal transduction pathways.