Exercise training increases CISD family protein expression in murine skeletal muscle and white adipose tissue
Exercise training increases CISD family protein expression in murine skeletal muscle and white adipose tissue
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运动训练增加小鼠骨骼肌和白色脂肪组织中 CISD 家族蛋白的表达
DOI：
10.1016/j.bbrc.2018.10.101
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发表时间：
2018
影响因子：
3.1
通讯作者：
Fujita Satoshi
中科院分区：
文献类型：
--
作者：
Yokokawa Takumi;Kido Kohei;Suga Tadashi;Sase Kohei;Isaka Tadao;Hayashi Tatsuya;Fujita Satoshi
骨骼肌和白色脂肪组织（WAT）中的线粒体功能随着年龄的增长以及2型糖尿病和胰岛素抵抗的进展而下降。虽然运动增加了两种组织中线粒体的生物发生和功能，但其分子机制尚未完全了解。CDGSH含铁硫结构域蛋白（CISDs）是一类新的调节线粒体活性和生物合成的蛋白质家族。然而，运动和CISD表达之间的关系尚不清楚。我们解决了这一问题，在本研究中，通过检查的表达变化的CISDs和线粒体蛋白在骨骼肌和WAT的小鼠进行慢性运动训练。将小鼠随机分配到久坐组或运动组，并分别在没有或有转轮的标准笼中饲养4周。免疫印迹法检测跖肌、比目鱼肌和附睾WAT中CISD和线粒体蛋白水平。慢性运动增加CISD1和CISD2以及线粒体蛋白表达在跖肌和WAT，但不是比目鱼肌。此外，这种运动诱导的适应与线粒体蛋白表达密切相关。因此，慢性运动诱导的线粒体生物合成与特定组织中CISDs的表达相一致，这可能是维持线粒体完整性的关键。
Mitochondrial function in skeletal muscle and white adipose tissue (WAT) declines with aging and the progression of type 2 diabetes and insulin resistance. Although exercise increases mitochondrial biogenesis and function in both tissues, the molecular mechanisms are not fully understood. CDGSH iron sulfur domain-containing proteins (CISDs) are a novel family of proteins that regulate mitochondrial activity and biogenesis. However, the relationship between exercise and CISD expression is unclear. We addressed this in the present study by examining changes in the expression of CISDs and mitochondrial proteins in skeletal muscle and WAT of mice subjected to chronic exercise training. Mice were randomly assigned to either the sedentary or exercise group and were housed for 4 weeks in a standard cage without or with a running wheel, respectively. CISD and mitochondrial protein levels in the plantaris and soleus muscles and epididymal WAT were evaluated by western blotting. Chronic exercise increased CISD1 and CISD2 as well as mitochondrial protein expression in plantaris muscle and WAT but not soleus muscle. Moreover, this exercise-induced adaptation was strongly correlated with mitochondrial protein expression. Thus, mitochondrial biogenesis induced by chronic exercise coincides with the expression of CISDs in specific tissues, which may be critical for the maintenance of mitochondrial integrity.