Development of an Enzyme-Linked Immunosorbent Assay for the α-Cyano Pyrethroids Multiresidue in Tai Lake Water
Development of an Enzyme-Linked Immunosorbent Assay for the α-Cyano Pyrethroids Multiresidue in Tai Lake Water
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DOI：
10.1021/jf803807b
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发表时间：
2009-04-22
影响因子：
6.1
通讯作者：
Xu, C. L.
中科院分区：
文献类型：
--
作者：
Hao, X. L.;Kuang, H.;Xu, C. L.
建立了一种检测α-氰基拟除虫菊酯的通用、宽类选择性竞争酶联免疫吸附试验（ELISA）。将一类特异性半抗原5-（3-苄基苯基）-5-氰基戊酸（CPBA-BE）与BSA偶联作为免疫原。产生多克隆抗体，PY-抗体。进一步优化了对α-氰基拟除虫菊酯家族（如苯氰菊酯）具有最大选择性的试验，并检测了对共溶剂、pH值和离子强度变化的耐受性。优化的免疫测定的IC 50值分别为4.58 μ g L-1的苯氰菊酯，5.62 μ g L-1的甲氰菊酯，7.08 μ g L-1的溴氰菊酯，10.72 μ g L-1的氯氰菊酯，19.95 μ g L-1的氟氰菊酯，和28.18 μ g L-1的高氰戊菊酯。对苯氰菊酯、甲氰菊酯、溴氰菊酯、氯氰菊酯、氟氰菊酯和顺式氰戊菊酯的交叉反应率分别为100%、81.49%、64.68%、42.72%、22.96%和16.25%。低检测限（LDL），本试验为0.107 μ g L-1苯氰菊酯。将该方法用于太湖水中α-氰基拟除虫菊酯的检测。
A general and broad class selective competitive enzyme-linked immunosorbent assay (ELISA) for the detection of the a-cyano pyrethroids was developed. One class specific hapten, the 5-(3-benzylphenyl)-5-cyanopentanoic acid (CPBA-BE), was conjugated with BSA as an immunogen. Polyclonal antibodies, PY-antibodies, were generated. The assay with the most selectivity for the family cc-cyano pyrethroids such as cyphenothrin was further optimized and tested for tolerance to cosolvent, pH, and ionic strength changes. The IC50 values of the optimized immunoassay were 4.58 mu g L-1 for cyphenothrin, 5.62 ug L-1 for fenpropathrin, 7.08 mu g L-1 for deltamethrin, 10.72 mu g L-1 for cypermethrin, 19.95 mu g L-1 for flucythrinate, and 28.18 mu g L-1 for esfenvalerate. The cross-reactivities of cyphenothrin, fenpropathrin, deltamethrin, cypermethrin, flucythrinate, and esfenvalerate were 100%, 81.49%, 64.68%, 42.72%, 22.96%, and 16.25%, respectively. The low detection limit (LDL) of this assay was 0.107 mu g L-1 for cyphenothrin. This method was used for the detection of the alpha-cyano pyrethroids in Tai lake water.