Adenovirus vector-mediated doxycycline-inducible RNA interference
Adenovirus vector-mediated doxycycline-inducible RNA interference
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DOI：
10.1089/1043034041648462
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发表时间：
2004-08-01
影响因子：
4.2
通讯作者：
Hayakawa, T
中科院分区：
文献类型：
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作者：
Hosono, T;Mizuguchi, H;Hayakawa, T
RNA干扰（RNAi）是一种抑制基因表达的有力工具。在这里，我们报告的发展腺病毒（Ad）载体介导的强力霉素（Dox）诱导的小干扰RNA（siRNA）表达系统。我们使用这种siRNA系统来控制人癌细胞中p53和c-Myc的表达。含有受Dox诱导型H1启动子控制的SiRNA表达系统的Ad载体和表达四环素阻遏物的Ad载体的共感染以剂量依赖性方式抑制了p53和c-Myc的表达水平，无论是Dox还是病毒剂量。获得了p53和c-Myc表达的调节沉默。由于Ad载体介导的可诱导RNAi系统可以在体外和体内有效地转染多种细胞类型，并且基因表达的缺失程度可以根据Dox的剂量进行调节，因此该表达系统应该是分析基因功能的基础研究和RNAi的治疗应用的有用工具。
RNA interference (RNAi) is a powerful tool for the knockdown of gene expression. Here, we report on the development of an adenovirus (Ad)vector-mediated doxycycline (Dox)-inducible small interfering RNA (siRNA) expression system. We used this siRNA system to control the expression of p53 and c-Myc in human cancer cells. Coinfection of Ad vectors containing the siRNA expression system under the control of the Dox-inducible H1 promoter and Ad vectors expressing a tetracycline repressor inhibited the expression levels of p53 and c-Myc in a dose-dependent manner with both Dox and viral dose. Regulated silencing of p53 and c-Myc expression was obtained. Because an Ad vector-mediated inducible RNAi system can efficiently transduce a variety of cell types in vitro and in vivo, and the degree of loss of gene expression can be modulated according to the dose of Dox, this expression system should be a useful tool for both basic research on the analysis of gene function and therapeutic applications of RNAi.