Biosynthesis and biodegradability of copolythioesters from 3,3′-thiodipropionic acid and plant oils by Cupriviadus necator
Biosynthesis and biodegradability of copolythioesters from 3,3′-thiodipropionic acid and plant oils by Cupriviadus necator
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DOI：
10.1002/mabi.200600239
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发表时间：
2007-03-08
影响因子：
4.6
通讯作者：
Endo, Takeshi
中科院分区：
文献类型：
--
作者：
Kamei, Yasutaka;Nagai, Atsushi;Endo, Takeshi
为了有效地制备含硫天然聚合物，多种植物油和3,3'-硫代二丙酸（TDP）被用作碳源，由野生型细菌Cupriviadus necator H16生物合成共聚物聚[（3-羟基丁酸酯）-共-（3-巯基丙酸酯）][聚（3HB-co-3MP）]。通过使用植物油，共聚物的积累和3MP单元的结合比使用糖的情况更多。通过调整培养条件，3MP 分数可控制在 1-39 mol-% 的范围内。已在活性污泥上清液中检查了共聚物的微生物降解性。生物降解通过两种机制进行：表面侵蚀和自动催化水解，具体取决于 3MP 单元分数，并显示出 3HB 单元序列的优先降解。
To prepare sulfur-containing natural polymers effectively, several plant oils and 3,3'-thiodipropionic acid (TDP) have been used as carbon sources for the biosynthesis of copolymer poly[ (3 -hydroxybutyrate)-co-(3-mercaptopropionate)] [poly(3HB-co-3MP)] by a wild-type bacterium Cupriviadus necator H16. By using the plant oils, copolymer accumulation and incorporation of 3MP units are greater than those of cases using sugars. The 3MP fraction is controllable over a range of 1-39 mol-% by adjusting the cultivation conditions. Microbial degradability of the copolymers has been examined in an activated sludge supernatant. The biodegradation proceeded by two mechanisms: surface erosion and auto-catalytic hydrolysis, depending on the 3MP unit fraction, and show preferential degradation of 3HB unit sequences.