Protein electrophoresis in agarose gels for separating high molecular weight proteins.
Protein electrophoresis in agarose gels for separating high molecular weight proteins.
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DOI：
10.1007/978-1-61779-821-4_10
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发表时间：
2012-01-01
期刊：
影响因子：
--
通讯作者：
Warren, Chad M
中科院分区：
文献类型：
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作者：
Greaser, Marion L;Warren, Chad M
很大的蛋白质(亚基大小为200 kDa)很难在聚丙烯酰胺凝胶上电泳法分离。一种SDS垂直琼脂糖凝胶系统已经被开发出来，它极大地提高了对非常大的蛋白质的分辨能力。分子量在200 kDa到4000 kDa之间的蛋白质可以清楚地分离出来。在上部储层缓冲液中加入还原剂已被发现是获得最佳分辨率的关键技术步骤。
Very large proteins (subunit sizes >200 kDa) are difficult to electrophoretically separate on polyacrylamide gels. A SDS vertical agarose gel system has been developed that has vastly improved resolving power for very large proteins. Proteins with molecular masses between 200 and 4,000 kDa can be clearly separated. Inclusion of a reducing agent in the upper reservoir buffer has been found to be a key technical procedure for obtaining optimum resolution.