Discovery of differentially expressed genes in cashmere goat (Capra hircus) hair follicles by RNA sequencing
Discovery of differentially expressed genes in cashmere goat (Capra hircus) hair follicles by RNA sequencing
复制标题
通过RNA测序发现绒山羊（Capra hircus）毛囊差异表达基因
DOI：
10.4238/gmr.15038589
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发表时间：
2016-01-01
影响因子：
0.4
通讯作者：
Li, J. Q.
中科院分区：
文献类型：
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作者：
Qiao, X.;Wu, J. H.;Li, J. Q.
哺乳动物毛囊是一种独特的、高度再生的器官，具有明显的发育周期。绒山羊(Capra Hircus)HFs按结构和发育时间可分为两类：初级卵泡和次级卵泡。为了确定绒山羊原发和继发HFs的差异表达基因(Deg)，对来自内蒙古阿尔巴斯的6个个体进行了RNA测序。共鉴定出617个deg；297个上调，320个下调。基因本体论分析表明，上调基因的主要功能是电子传递、呼吸电子传递、线粒体电子传递和基因表达。下调的基因主要涉及细胞自噬、蛋白质复合体、中性粒细胞聚集和细菌真菌防御反应。根据京都百科全书的基因和基因组数据库，这些基因主要参与半胱氨酸和蛋氨酸的代谢、RNA聚合和MAPK信号通路，并在初级卵泡中丰富。MicroRNA靶标网络揭示了次级毛囊参与了几个重要的生物学过程，如角蛋白相关蛋白的合成和参与氨基酸生物合成的酶的合成。综上所述，这些发现将加深我们对HF发育和循环的复杂分子机制的理解，并为进一步研究HF发育的基因和功能提供基础。
The mammalian hair follicle (HF) is a unique, highly regenerative organ with a distinct developmental cycle. Cashmere goat (Capra hircus) HFs can be divided into two categories based on structure and development time: primary and secondary follicles. To identify differentially expressed genes (DEGs) in the primary and secondary HFs of cashmere goats, the RNA sequencing of six individuals from Arbas, Inner Mongolia, was performed. A total of 617 DEGs were identified; 297 were upregulated while 320 were downregulated. Gene ontology analysis revealed that the main functions of the upregulated genes were electron transport, respiratory electron transport, mitochondrial electron transport, and gene expression. The downregulated genes were mainly involved in cell autophagy, protein complexes, neutrophil aggregation, and bacterial fungal defense reactions. According to the Kyoto Encyclopedia of Genes and Genomes database, these genes are mainly involved in the metabolism of cysteine and methionine, RNA polymerization, and the MAPK signaling pathway, and were enriched in primary follicles. A microRNA-target network revealed that secondary follicles are involved in several important biological processes, such as the synthesis of keratin-associated proteins and enzymes involved in amino acid biosynthesis. In summary, these findings will increase our understanding of the complex molecular mechanisms of HF development and cycling, and provide a basis for the further study of the genes and functions of HF development.