Effects of zinc deficiency and supplementation on gene expression of bitter taste receptors (TAS2Rs) on the tongue in rats
Effects of zinc deficiency and supplementation on gene expression of bitter taste receptors (TAS2Rs) on the tongue in rats
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DOI：
10.1002/lary.23378
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发表时间：
2012-11-01
期刊：
影响因子：
2.6
通讯作者：
Ikeda, Minoru
中科院分区：
文献类型：
--
作者：
Sekine, Hiroki;Takao, Kyoichi;Ikeda, Minoru
目的/假设：研究缺锌和补锌对大鼠舌上苦味受体（TAS2R）和上皮钠离子通道（ENaC）基因表达的影响。研究设计：前瞻性动物研究。方法：选用3周龄雄性Sprague-Dawley大鼠36只。12只大鼠分别饲喂28 （n = 6）或56 （n = 6）天的正常饮食，12只大鼠饲喂缺锌饮食，12只大鼠饲喂28天的缺锌饮食，再饲喂28天的锌饮食。采集环瓣乳头上皮，采用逆转录-聚合酶链反应检测TAS2R40、TAS2R105、TAS2R107、TAS2R118、TAS2R121、TAS2R136、TAS2R140和ENaC基因的表达。结果：缺锌大鼠TAS2R40和TAS2R107基因表达频率显著降低，给锌后TAS2R107基因表达频率显著升高。在缺锌大鼠中，TAS2R105、TAS2R118、TAS2R121或ENaC的表达未见变化。结论：我们的研究结果表明锌可能影响大鼠舌上一些TAS2Rs基因的表达。喉镜,2012
Objectives/Hypothesis: We examined the effect of zinc deficiency and supplementation on the expression of bitter taste receptor (TAS2R) and epithelial sodium ion channel (ENaC) genes on the tongue in rats. Study Design: Prospective animal study. Methods: A total of 36 male Sprague-Dawley rats (3 weeks old) were used. Twelve rats were fed a normal diet for 28 (n = 6) or 56 (n = 6) days, another 12 were fed a zinc-deficient diet for the same periods, and still another 12 were fed a zinc-deficient diet for 28 days and then administered zinc for a further 28 days. The epithelium of the circumvallate papillae was collected, and expression of the TAS2R40, TAS2R105, TAS2R107, TAS2R118, TAS2R121, TAS2R136, TAS2R140, and ENaC genes was examined by reverse transcriptase-polymerase chain reaction. Results: Gene expression frequency of TAS2R40 and TAS2R107 significantly decreased in rats fed a zinc-deficient diet, and frequency of TAS2R107 significantly increased following zinc administration. No changes were noted in expression of TAS2R105, TAS2R118, TAS2R121, or ENaC in zinc-deficient rats. Conclusions: Our findings suggest that zinc may affect gene expression for some TAS2Rs on the tongue in rats. Laryngoscope, 2012