INFLUENCE OF ENVIRONMENTAL-FACTORS UPON THE SURVIVAL OF AUJESZKYS DISEASE VIRUS
INFLUENCE OF ENVIRONMENTAL-FACTORS UPON THE SURVIVAL OF AUJESZKYS DISEASE VIRUS
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DOI：
10.1016/s0034-5288(18)32516-5
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发表时间：
1981-01-01
影响因子：
2.4
通讯作者：
BERAN, GW
中科院分区：
文献类型：
--
作者：
DAVIES, EB;BERAN, GW
研究发现伪狂犬病病毒在活宿主外的存活取决于pH值和温度。在4 ℃下，病毒灭活的速率为0.04 log/天。在37 ℃下，从0.6log/天增加到0.6log/天。最适pH值为6 - 8。温度在4和37 ℃之间波动。C对病毒滴度无明显影响。在-13 ℃的稳定温度下，C，病毒在所有测试的pH水平下快速灭活。对于长期储存，建议pH应调节至6 - 7，悬浮液应保持在-90 ℃。冷冻和解冻应该迅速，以便尽快通过零度以下的温度区。在玻璃和明胶上干燥，导致病毒在所有pH水平下灭活，暴露于紫外线下也是如此。
The survival of Aujeszky''s disease (pseudorabies) virus outside the living host was found to be dependant on pH level and temperature. Virus inactivation occurred at a rate varying from 0.04 log/day at 4.degree. C to 0.6 log/day at 37.degree. C at the optimum pH levels of 6-8. Fluctuation of the temperature between 4 and 37.degree. C had no apparent effect on the virus titer. At a steady temperature of -13.degree. C, the virus was rapidly inactivated at all tested pH levels. For long-term storage it is recommended that the pH should be adjusted to 6-7, that suspensions should be kept at -90.degree. C and that freezing and thawing should be rapid so as to pass through the just subzero temperature zone as quickly as possible. Drying, on glass and gelatin, caused inactivation of the virus at all pH levels, as did exposure to UV light.