The effect of methyl salicylate on the induction of direct and indirect plant defense mechanisms in poplar (Populus x euramericana 'Nanlin 895')
The effect of methyl salicylate on the induction of direct and indirect plant defense mechanisms in poplar (Populus x euramericana 'Nanlin 895')
复制标题
水杨酸甲酯对杨树（杨树‘南林895’）直接和间接植物防御机制诱导的影响
DOI：
10.1080/17429145.2015.1020024
复制
发表时间：
2015
影响因子：
3.2
通讯作者：
Ye Jian-Ren
中科院分区：
文献类型：
--
作者：
Tang Fang;Fu Ying-Ying;Ye Jian-Ren
研究了不同浓度水杨酸甲酯（梅萨）对欧美杨（Populus×euramericana'Nanlin 895'）白杨苗直接防御和间接防御的影响。测定了超氧化物歧化酶（SOD，EC1.15.1.1）、氧化还原酶过氧化物酶（POD，EC1.11.1.7）、过氧化氢酶（CAT，EC1.11.1.6）和多酚氧化酶（PPO，EC 1.14.18.1）等4种防御相关酶的活性。结果表明：1.0和10.0mM梅萨对SOD活性有诱导作用，对POD活性有诱导作用。CAT活性在低浓度的梅萨诱导，但被抑制高浓度，和PPO活性的影响由0.1，1.0，和10.0 mM的梅萨。此外，挥发性物质进行了检测，使用气相色谱-质谱和气相色谱。初步鉴定出21种挥发性化合物。在这些挥发性化合物中，（Z）-3-己烯-1-醇和顺式-3-己烯基乙酸酯的排放量最高。（Z）-3-壬烯-1-醇、（E）-2-己烯-1-醇、1-辛醇、（E，Z）-3，6-壬二烯-1-醇、β-紫罗兰酮和十六酰胺是仅在10.0 mM梅萨处理后产生的六种化合物。这些结果表明梅萨诱导了杨树的直接和间接防御机制。
The objective of this study was to investigate the effect of different concentrations of methyl salicylate (MeSA) on direct defense and indirect defense in poplar cuttings (Populus×euramericana‘Nanlin 895’). Four defense-related enzyme activities, such as superoxide dismutase (SOD, EC1.15.1.1), oxydoreductases peroxidase (POD, EC1.11.1.7), catalase (CAT, EC1.11.1.6), and polyphenol oxidase (PPO, EC 1.14.18.1), were measured. The results showed that the SOD activities were induced by 1.0 and 10.0 mM MeSA and the POD activities were induced by MeSA. The CAT activity was induced at low concentrations of MeSA but was inhibited by high concentrations, and the PPO activity was affected by 0.1, 1.0, and 10.0 mM MeSA. Furthermore, the volatiles were detected using gas chromatography–mass spectrometry and gas chromatography. Twenty-one volatile compounds were tentatively identified in the emissions from plant leaves. Of these volatile compounds, (Z)-3-hexen-1-ol andcis-3-hexenyl acetate emissions were the highest. (Z)-3-Nonen-1-ol, (E)-2-hexen-1-ol, 1-octanol, (E,Z)-3,6-nonadien-1-ol,β-ionone, and hexadecanamide were six compounds that were only produced after treatment by 10.0 mM MeSA. These results showed that direct and indirect defense mechanisms in poplars were induced by MeSA.