Arabidopsis thaliana as a model system for graft union development in homografts and heterografts
Arabidopsis thaliana as a model system for graft union development in homografts and heterografts
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DOI：
10.1007/s00344-008-9050-y
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发表时间：
2008-09-01
影响因子：
4.8
通讯作者：
Lev-Yadun, Simcha
中科院分区：
文献类型：
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作者：
Flaishman, Moshe A.;Loginovsky, Kamelia;Lev-Yadun, Simcha
以拟南芥为砧木，进行了拟南芥接穗的嫁接研究。以拟南芥为材料，研究了嫁接体的亲和性和嫁接体形成的个体发育。以3个月大的A.拟南芥植物。组织学分析揭示了拟南芥同源嫁接中嫁接愈合形成的四个发育阶段：（1）坏死层的发育，（2）嫁接接穗中愈伤组织的增殖，（3）接穗内新维管组织的分化，和（4）接穗和砧木之间完全维管嫁接愈合的形成。嫁接后15天，砧木和接穗之间的愈伤组织桥内形成了维管连接。拟南芥异源嫁接在甘蓝科（Brassica）和萝卜科（Raphanus）的两个成员上，表现出部分不相容的相互作用，维管分化水平较低。拟南芥嫁接番茄（茄科）砧木表现出完全的不相容性和有限的不连续分化的新的维管组织，没有跨越接穗/股票边界。虽然缺乏接穗/股票维管连接，拟南芥接穗嫁接到番茄砧木开花，产生种子。这可能表明两种植物之间的一些非维管功能连接，可能是薄壁细胞，进一步强调拟南芥作为模型植物的有用性，用于研究各种水平的复杂的接穗/股票的关系，在嫁接生物学。染料运输在木质部的实验表明，虽然在一般情况下有一个协议之间的组织学研究和染料运输，在拟南芥同源移植水运输频率低于功能和组织相容性。我们的结论是，同源嫁接和异源嫁接的拟南芥花序茎是一个方便和重复性的方法，研究嫁接生物学的基本细胞遗传和分子方面。
Homografting of Arabidopsis thaliana scions on stocks of A. thaliana and heterografting on other species were used to study the compatibility and the ontogeny of graft union formation. Highly compatible homografting with scions of young leafy inflorescence stems was obtained on stocks of inflorescence stems growing from large 3-month-old A. thaliana plants. Histologic analysis revealed four developmental stages of graft union formation in Arabidopsis homografting: (1) development of a necrotic layer, (2) callus proliferation in the grafted scion, (3) differentiation of new vascular tissues within the scion, and (4) a full vascular graft union formation between the scion and the stock. Vascular connections were formed within the callus bridge between rootstocks and scions 15 days after grafting. Heterografts of Arabidopsis on two members of Brassicaceae, cabbage (Brassica) and radish (Raphanus), showed partial incompatible interaction with a lower level of vascular differentiation. Arabidopsis grafting on tomato (Solanaceae) rootstock showed complete incompatibility and limited noncontinuous differentiation of new vascular tissues that did not cross the scion/stock boundary. Although lacking scion/stock vascular connections, Arabidopsis scions grafted onto tomato rootstock flowered and produced seeds. This may indicate some nonvascular functional connections between the two plants, probably of parenchyma cells, further emphasizing the usefulness of Arabidopsis as a model plant for studying various levels of the complicated scion/stock relationships expressed in grafting biology. Experiments with dye transport in the xylem showed that although in general there was an agreement between the histologic study and dye transport, in Arabidopsis homografts water transport frequency was lower than functional and histologic compatability. We conclude that homografting and heterografting of Arabidopsis inflorescence stems is a convenient and reproducible method for studying the fundamental cellular genetic and molecular aspects of grafting biology.