Recent advances in genomic DNA sequencing of microbial species from single cells.

Recent advances in genomic DNA sequencing of microbial species from single cells.
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DOI:
10.1038/nrg3785
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发表时间:
2014-09
期刊:
Nature reviews. Genetics
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绝大多数微生物物种仍未培养,直到最近,大约一半的已知细菌门仅通过其 16S 核糖体 RNA 基因序列进行鉴定。随着单细胞测序的出现,未栽培物种的基因组正在迅速填充微生物系统发育树中未测序的分支。从这些以前无法接触到的群体中获得的大量新见解,使人们能够更深入地了解它们的基础生物学、分类学和进化,以及它们在环境生态系统和人类健康中的不同作用。
The vast majority of microbial species remain uncultivated and, until recently, about half of all known bacterial phyla were identified only from their 16S ribosomal RNA gene sequence. With the advent of single-cell sequencing, genomes of uncultivated species are rapidly filling in unsequenced branches of the microbial phylogenetic tree. The wealth of new insights gained from these previously inaccessible groups is providing a deeper understanding of their basic biology, taxonomy and evolution, as well as their diverse roles in environmental ecosystems and human health.
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