Localization and sex-difference of steroid receptor coactivator-1 immunoreactivities in the brain of adult female and male mice
Localization and sex-difference of steroid receptor coactivator-1 immunoreactivities in the brain of adult female and male mice
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DOI：
10.1016/j.steroids.2010.11.009
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发表时间：
2011-02-01
期刊：
影响因子：
2.7
通讯作者：
Zhang, Jiqiang
中科院分区：
文献类型：
--
作者：
Bian, Chen;Zhang, Dongmei;Zhang, Jiqiang
女性和男性在大脑和行为上是不同的。这些差异是由类固醇及其核受体介导的，需要辅激活子来调节靶基因的转录。研究表明，这些辅激活因子对调节大脑中类固醇激素的作用至关重要。类固醇受体辅激活因子-1（SRC-1）在生殖、应激、运动学习等方面具有重要的调节作用，且SRC-1 mRNA或蛋白在特定脑区的表达存在性别差异，但SRC-1在成年男女脑中的表达及其差异尚不清楚。在这项研究中，我们报告说，在两种性别，高水平的SRC-1免疫反应性检测嗅球，大脑皮层，海马，浦肯野细胞，一些有限的间脑和脑干核。免疫阳性物质主要在细胞核中检测到，但在某些区域，它们也在突起或纤维样结构中检测到。在大多数的大脑区域研究，男性具有显着较高的SRC-1免疫反应性水平比女性。在一些与学习和记忆、运动调节和生殖相关的核团中检测到较高水平的SRC-1，表明其在神经退行性变和性别依赖性行为和结构中的潜在作用; SRC-1免疫反应的区域和性别特异性定位与某些类固醇受体的定位一致，表明该辅激活因子在这些类固醇反应区域和与生殖有关的细胞群中发挥重要作用，学习和记忆，运动和感觉的整合。(C)2010年爱思唯尔公司All rights reserved.
Females and males are different in brain and behaviors. These differences are mediated by steroids and their nuclear receptors which require coactivators to regulate the transcription of target genes. Studies have shown that these coactivators are critical for modulating steroid hormone action in the brain. Steroid receptor coactivator-1 has been implied in the regulation of reproduction, stress, motor learning, and limited studies have reported the sex-specific difference of SRC-1 mRNA or protein expression in specific brain regions, but the expression and differences of SRC-1 immunoreactivities in adult female and male brain remain unclear. In this study we reported that in both sexes, high levels of SRC-1 immunoreactivities were detected in olfactory bulb, cerebral cortex, hippocampus, Purkinje cells, some limited diencephalon and brainstem nuclei. The immunopositive materials were predominantly detected in cell nucleus, but in some regions they were also detected in the processes or fiber-like structures. In most of the brain regions studied, males possessed significantly higher levels of SRC-1 immunoreactivities than that of females. Higher levels of SRC-1 were detected in some nuclei related to learning and memory, motor regulation and reproduction indicated its potential roles in neurodegeneration and sex-dependent behavior and structure; the region- and sex-specific localization of SRC-1 immunoreactivities in agreement with that of some steroid receptors, indicating this coactivator play important roles in these hormone-reactive regions and cell groups related to reproduction, learning and memory, integration of motor and sense. (C) 2010 Elsevier Inc. All rights reserved.