FLUORESCENT HYDRAZIDES FOR THE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF BIOLOGICAL CARBONYLS
FLUORESCENT HYDRAZIDES FOR THE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF BIOLOGICAL CARBONYLS
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DOI：
10.1016/0003-2697(86)90133-8
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发表时间：
1986-01-01
影响因子：
2.9
通讯作者：
ANDERSON, JM
中科院分区：
文献类型：
--
作者：
ANDERSON, JM
采用新型荧光衍生试剂对高效液相色谱法测定生物源羰基化合物的方法进行了改进。合成了8种荧光肼，并与丹酰肼（1-二甲氨基萘-5-磺酰肼）进行了比较。在乙腈-水混合物中，四种化合物产生的羰基腙的相对荧光量子产率高于丹酰肼。在测定植物组织中的脱落酸和茉莉酸时，Darpsyl肼[（3-苯基吡唑啉-1-酰基）-4-苯基磺酰肼]和apmayl肼[N-（2-氨基苯基-6-甲基苯并噻唑）-乙酰肼]的灵敏度都比danyl肼提高了20倍以上。不同的酰肼和衍生化条件是测定不同羰基化合物的最佳条件。此外，开发了一种用于柱前纯化酸性羰基腙的简单方法，以去除衍生化过程中产生的污染物和来自组织源的污染物。
Methods for the determination of carbonyl compounds of biological origin by high-performance liquid chromatography were improved by the use of new fluorescent derivatizing agents. Eight fluorescent hydrazides were either synthesized or obtained commercially and compared to dansyl hydrazine (1-dimethylaminonaphthalene-5-sulfonylohydrazide). Four of the compounds yielded carbonyl hydrazones with a higher relative fluorescence quantum yield than dansyl hydrazine in acetonitrile:water mixtures. Darpsyl hydrazide [(3-phenylpyrazoline-1-yl)-4-phenylsulfonylohydrazide] and apmayl hydrazide [N-(2-aminophenyl-6-methylbenzthiazole)-acetylohydrazide] both yielded an increase of greater than 20-fold in sensitivity over dansyl hydrazine in determinations of abscisic acid and jasmonic acid from plant tissues. Different hydrazides and derivatizing conditions were found to be optimum for the determination of different carbonyl compounds. Also, a simple method for precolumn purification of the hydrazones of acidic carbonyls was developed to remove contaminants arising during derivatization and from the tissue source.