3D Printed Microfluidic Devices for Solid-Phase Extraction and On-Chip Fluorescent Labeling of Preterm Birth Risk Biomarkers
3D Printed Microfluidic Devices for Solid-Phase Extraction and On-Chip Fluorescent Labeling of Preterm Birth Risk Biomarkers
复制标题
DOI：
10.1021/acs.analchem.0c01970
复制
发表时间：
2020-09-15
影响因子：
7.4
通讯作者：
Woolley, Adam T.
中科院分区：
文献类型：
--
作者：
Bickham, Anna, V;Pang, Chao;Woolley, Adam T.
固相萃取（SPE）是一种用于样品制备的通用预浓缩方法，可用于各种样品。3D打印微流体装置内SPE的小型化进一步允许快速、简单地提取分析物，同时还能够将SPE与其他样品制备和分离方法集成。在这里，我们介绍了反相甲基丙烯酸月桂酯基整料的开发和应用，该整料在3D打印的微流体设备中形成，可以选择性地保留肽和蛋白质。这些SPE整料和3D打印微流体装置的有效性使用一组9种不同疏水性的早产生物标志物进行测试，质量范围为2至470 kDa。生物标记物被选择性地保留，荧光标记，并在3D打印的微流体系统中与过量的荧光标记分开洗脱。这是第一个结果，展示了一个完整的早产生物标志物面板上的微流体分析过程，这是朝着开发小型化，完全集成的分析系统迈出的重要一步。
Solid-phase extraction (SPE) is a general preconcentration method for sample preparation that can be performed on a variety of specimens. The miniaturization of SPE within a 3D printed microfluidic device further allows for fast and simple extraction of analytes while also enabling integration of SPE with other sample preparation and separation methods. Here, we present the development and application of a reversed-phase lauryl methacrylate-based monolith, formed in 3D printed microfluidic devices, which can selectively retain peptides and proteins. The effectiveness of these SPE monoliths and 3D printed microfluidic devices was tested using a panel of nine preterm birth biomarkers of varying hydrophobicities and ranging in mass from 2 to 470 kDa. The biomarkers were selectively retained, fluorescently labeled, and eluted separately from the excess fluorescent label in 3D printed microfluidic systems. These are the first results demonstrating microfluidic analysis processes on a complete panel of preterm birth biomarkers, an important step toward developing a miniaturized, fully integrated analysis system.