Significant sensitivity improvements by matrix optimization: a MALDI-TOF mass spectrometric study of lipids from hen egg yolk
Significant sensitivity improvements by matrix optimization: a MALDI-TOF mass spectrometric study of lipids from hen egg yolk
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DOI：
10.1016/j.chemphyslip.2010.04.005
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发表时间：
2010-06-01
影响因子：
3.4
通讯作者：
Jaskolla, Thorsten W.
中科院分区：
文献类型：
--
作者：
Teuber, Kristin;Schiller, Juergen;Jaskolla, Thorsten W.
基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱（MS）由于其灵敏度、对杂质的耐受性和性能的简单性而越来越多地用于分析来自生物来源的脂质。虽然其详细的作用是不了解到目前为止，所应用的矩阵有一个显着的影响，可实现的光谱质量，特别是如何敏感的个别脂质类是可检测的。最近在脂质领域建立了不同的基质化合物，包括2，5-二羟基苯甲酸（DHB）、9-氨基吖啶（9-AA）、对硝基苯胺（PNA）、2-巯基苯并噻唑（MBT）和2-（2-氨基乙氨基）-5-硝基吡啶（AAN）。本文的目的是比较这些矩阵的性能与新合成的矩阵，α-氰基-2，4-二氟肉桂酸（二FCCA）。从鸡蛋黄的有机提取物被用作一个简单和容易获得的测试system.It将被证明，二FCCA是选择的矩阵，以检测脂质在正离子模式由于可实现的灵敏度增益超过一个数量级相比，替代矩阵。相反，Di-FCCA不适合磷脂的负离子检测。在这里，9-AA无疑是选择的矩阵。(C)2010爱思唯尔爱尔兰有限公司版权所有。
Due to its sensitivity, the tolerance of impurities and the simplicity of performance, matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) is increasingly used to analyze lipids from biological sources. Although its detailed role is not understood so far, the applied matrix has a pronounced effect on the achievable spectrum quality and particularly how sensitive the individual lipid classes are detectable. Different matrix compounds were recently established in the lipid field including 2,5-dihydroxybenzoic acid (DHB), 9-aminoacridine (9-AA), para-nitroaniline (PNA), 2-mercaptobenzothiazole (MBT), and 2-(2-aminoethylamino)-5-nitropyridine (AAN). It is the aim of this paper to compare the properties of these matrices with the newly synthesized matrix, alpha-cyano-2,4-difluorocinnamic acid (Di-FCCA). An organic extract from hen egg yolk was used as a simple and easily available test system.It will be shown that Di-FCCA is the matrix of choice to detect lipids in the positive-ion mode due to an achievable sensitivity gain of more than one order of magnitude compared to alternative matrices. In contrast, Di-FCCA is not suitable for negative-ion detection of phospholipids. Here, 9-AA is unequivocally the matrix of choice. (C) 2010 Elsevier Ireland Ltd. All rights reserved.