Quantitative recovery of pulmonary intravascular macrophages from sheep lungs.
Quantitative recovery of pulmonary intravascular macrophages from sheep lungs.
复制标题
从绵羊肺中定量回收肺血管内巨噬细胞。
DOI：
10.1002/jlb.56.6.692
复制
发表时间：
1994
影响因子：
5.5
通讯作者：
Brain,JD
中科院分区：
文献类型：
--
作者：
Rogers,RA;Tasat,DR;Warner,AE;Brain,JD
肺血管内巨噬细胞(PIM)附着在肺毛细血管内皮细胞上，隔离血液中的循环颗粒和病原体。氧化铁(γFe203)5毫克/公斤，静脉注射，在活体绵羊体内原位特异性标记PIM。使用血管灌流(VP)程序分离γFE203标记的PIM的尝试产生的细胞很少。为了提高PIMs的回收率，开发了一种蛋白分解肺消化(PLD)方法。在PLD后，用磁铁回收含有γFe203的PIM，并用磁通门磁强计测量γFe203的含量。肺组织蛋白水解液可回收肺组织总γFe203的34%，每克肺可获得2105个PIMs，存活率为95%。相比之下，VP只回收了肺中总γFe203的3%，而且回收的γFe203中只有不到2%是细胞相关的。蛋白水解性肺消化和磁分离是一种回收绵羊PIM活性以进行体外研究的有效方法。比奥尔。1994年：56：692-701。
Pulmonary intravascular macrophages (PIMs) adhere to the endothelium of lung capillaries and sequester circulating particles and pathogens from the blood. Iron oxide (γFe203) 5 mg/kg, administered intravenously, specifically labeled PIMs in situ within the living sheep. Attempts to isolate γFe203-labeled PIMs using vascular perfusion (VP) procedures yielded few cells. To improve recovery of PIMs, a proteolytic lung digestion (PLD) procedure was developed. Following PLD, γFe203-containing PIMs were recovered by magnets and the amount of γFe203present measured by fluxgate magnetometry. Proteolytic lung digestion recovered 34% of the total γFe203in lung samples and yielded 2 105PIMs/g lung with 95% viability. In contrast, VP recovered only 3% of the total γFe203in the lung; furthermore, less than 2% of the recovered γFe203was cell associated. Proteolytic lung digestion followed by magnetic separation is an effective way to recover viable sheep PIMs for in vitro study.J. Leukoc. Biol. 56: 692–701; 1994.