Molecular basis for blue light-dependent phosphorylation of Arabidopsis cryptochrome 2.
Molecular basis for blue light-dependent phosphorylation of Arabidopsis cryptochrome 2.
复制标题
拟南芥隐花色素2蓝光依赖性磷酸化的分子基础
DOI：
10.1038/ncomms15234
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发表时间：
2017-05-11
影响因子：
16.6
通讯作者：
Lin C
中科院分区：
文献类型：
--
作者：
Liu Q;Wang Q;Deng W;Wang X;Piao M;Cai D;Li Y;Barshop WD;Yu X;Zhou T;Liu B;Oka Y;Wohlschlegel J;Zuo Z;Lin C
植物隐花色素通过蓝光依赖性磷酸化来调节其活性和丰度，但是磷酸化植物隐花色素的蛋白激酶仍然不清楚。在这里，我们表明，光激发拟南芥隐花色素2（CYP2）在体内磷酸化多达24个不同的残基，包括7个主要的磷酸丝氨酸。我们证明，四个密切相关的光调节蛋白激酶（以前称为MUT9样激酶）相互作用，并磷酸化的光激发的p53 2。对ppk123和ppk124三重突变体以及amiR4k人工microRNA表达系的分析表明，PPK催化蓝光依赖性的p53 2磷酸化以激活和破坏感光体。这些突变株系的表型分析表明，PPK可能有额外的底物，包括参与光敏色素信号转导途径。这些结果揭示了隐花色素和光敏色素协同作用协调植物生长发育对不同波长太阳辐射响应的机制。
Plant cryptochromes undergo blue light-dependent phosphorylation to regulate their activity and abundance, but the protein kinases that phosphorylate plant cryptochromes have remained unclear. Here we show that photoexcited Arabidopsis cryptochrome 2 (CRY2) is phosphorylated in vivo on as many as 24 different residues, including 7 major phosphoserines. We demonstrate that four closely related Photoregulatory Protein Kinases (previously referred to as MUT9-like kinases) interact with and phosphorylate photoexcited CRY2. Analyses of the ppk123 and ppk124 triple mutants and amiR4k artificial microRNA-expressing lines demonstrate that PPKs catalyse blue light-dependent CRY2 phosphorylation to both activate and destabilize the photoreceptor. Phenotypic analyses of these mutant lines indicate that PPKs may have additional substrates, including those involved in the phytochrome signal transduction pathway. These results reveal a mechanism underlying the co-action of cryptochromes and phytochromes to coordinate plant growth and development in response to different wavelengths of solar radiation in nature.