Hormone and phenol levels during germination and osmopriming of tomato seeds, and associated variations in protein patterns and anatomical seed features
Hormone and phenol levels during germination and osmopriming of tomato seeds, and associated variations in protein patterns and anatomical seed features
复制标题
DOI：
10.1556/aagr.54.2006.4.7
复制
发表时间：
2006-12-01
期刊：
影响因子：
--
通讯作者：
Hegazi, A. Z. A.
中科院分区：
文献类型：
--
作者：
El-Araby, M. M.;Moustafa, S. M. A.;Hegazi, A. Z. A.
番茄（Lycopersicon escidentum Mill.，品种城堡岩）在聚乙二醇6000（PEG; 20%）或K2 HPO 4（200 mM）溶液中预处理8小时、3天或7天，而另一组种子在水中放置相同的时间。GA（3）/阿坝比值是促进H2O浸泡种子萌发的最重要的激素因子，从而提高了种子的发芽率。该比率在8小时后在水引发的和水引发的种子之间显示出轻微的变化，但是之后，从3至7天，它在水引发的种子中逐渐增加，并且在H2O中发芽的种子中显著升高。酚类化合物的浓度的变化表明其可能的作用，在发芽沉默的预处理的种子，但在相对较低的浓度。蛋白质模式没有显着的变化，在水引发和水引发种子后8小时，但观察到不同的类型，特别是在7天后。比较种子在PEG或K_2HPO_4、H_2O、GA（3）或阿坝处理1天和7天后的蛋白质带型，显示某些处理特异性蛋白质带，特别是在PEG和阿坝溶液中。种子的纵向切片（3天后）显示，在H2O或GA（3）中珠孔胚乳溶解和胚根突出，而在PEG或K2 HPO 4溶液中胚根在种子内膨胀，珠孔胚乳完全完整。在阿坝溶液中，整个胚乳致密，种子广泛脱水。
Seeds of tomato (Lycopersicon escidentum Mill., variety Castle Rock) were osmoprimed in polyethylene glycol 6000 (PEG; 20%) or K2HPO4 (200 mM) solution for 8 hours, 3 days or 7 days, while another group of seeds were left in water for the same periods. The GA(3)/ABA ratio was the most important hormone factor, which promoted germination in seeds soaked in H2O and led to improved germination performance. This ratio showed slight variations between hydroprimed and osmoprimed seeds after 8 hours, but afterwards, from 3 to 7 days, it was gradually increased in the osmoprimed seeds and was substantially elevated in seeds germinating in H2O. Changes in the concentrations of phenolic compounds suggested their possible role in germination silencing in the osmoprimed seeds, but at relatively low concentrations. Protein patterns showed no marked variations in hydroprimed and osmoprimed seeds after 8 hours, but different types were observed, particularly after 7 days. A comparison of the protein banding patterns of seeds after 1 day and 7 days in the osmoconditioning solutions (PEG or K2HPO4), H2O, GA(3) or ABA showed certain treatment-specific protein bands, particularly in PEG and ABA solutions. Longitudinal sections of seeds (after 3 days) showed lysis of the micropylar endosperm and radicle protrusion in H2O or GA(3), whereas in PEG or K2HPO4 solution the radicle expanded inside the seed and the micropylar endosperm was completely intact. In ABA solution, the whole endosperm was compact and the seed became extensively desiccated.