Isolation of vascular smooth muscle cells from a single murine aorta.

Isolation of vascular smooth muscle cells from a single murine aorta.
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DOI:
10.1023/a:1016357510143
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发表时间:
2001
期刊:
Methods in cell science : an official journal of the Society for In Vitro Biology
影响因子:
--
通讯作者:
Jenna Lynn Ray;Rebecca L. Leach;J. Herbert;Mark L. Benson
Jenna Lynn Ray;Rebecca L. Leach;J. Herbert;Mark L. Benson
中科院分区:
其他
文献类型:
--
作者:
Jenna Lynn Ray;Rebecca L. Leach;J. Herbert;Mark L. Benson

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The vascular smooth muscle cell plays a significant role in many important cardiovascular disorders, and smooth muscle biology is therefore important to cardiovascular research. The mouse is critical to basic cardiovascular research, largely because techniques for genetic manipulation are more fully developed in the mouse than in any othermammalian species. We describe here a technique for isolating smooth muscle cells from a single mouse aorta. This technique is particularly useful when material is limiting, as is frequently the case when genetically modified animals are being characterized.