Poplar acetylome profiling reveals lysine acetylation dynamics in seasonal bud dormancy release
Poplar acetylome profiling reveals lysine acetylation dynamics in seasonal bud dormancy release
复制标题
DOI：
10.1111/pce.14040
复制
发表时间：
2021-03-26
影响因子：
7.3
通讯作者：
Ding, Jihua
中科院分区：
文献类型：
--
作者：
Liao, Xiaoli;Li, Yue;Ding, Jihua
对于北方和温带生态系统中的多年生植物来说，芽休眠对于在严酷的冬季生存至关重要。休眠是通过长时间暴露在低温下而解除的，然后在春天进行反应性生长。赖氨酸乙酰化（Kac）是植物对环境信号应答的主要翻译后修饰（PTM）之一。然而，很少有资料是关于Kac修饰对芽休眠解除的影响。本文报道了山杨×银杨杂种休眠芽中赖氨酸乙酰组的动态变化。从3，281个乙酰基蛋白中鉴定了总共7，594个乙酰基位点，代表了植物中赖氨酸乙酰基组的大数据集。其中，229个蛋白质在芽休眠解除过程中发生了差异乙酰化，主要参与初级代谢途径。定点突变酶法分析表明，Kac强烈修改的两个主要代谢的关键酶，丙酮酸脱氢酶（PDH）和异柠檬酸脱氢酶（IDH）的活动。因此，我们建议，Kac的酶可能是一个重要的战略重构代谢过程中芽休眠解除。总之，我们的研究结果揭示了Kac在芽休眠解除中的重要性，并为理解树木季节性生长的分子机制提供了新的视角。
For perennials in boreal and temperate ecosystems, bud dormancy is crucial for survival in harsh winter. Dormancy is released by prolonged exposure to low temperatures and is followed by reactive growth in the spring. Lysine acetylation (Kac) is one of the major post-translational modifications (PTMs) that are involved in plant response to environmental signals. However, little information is available on the effects of Kac modification on bud dormancy release. Here, we report the dynamics of lysine acetylome in hybrid poplar (Populus tremula x Populus alba) dormant buds. A total of 7,594 acetyl-sites from 3,281 acetyl-proteins were identified, representing a large dataset of lysine acetylome in plants. Of them, 229 proteins were differentially acetylated during bud dormancy release and were mainly involved in the primary metabolic pathways. Site-directed mutagenesis enzymatic assays showed that Kac strongly modified the activities of two key enzymes of primary metabolism, pyruvate dehydrogenase (PDH) and isocitrate dehydrogenase (IDH). We thus propose that Kac of enzymes could be an important strategy for reconfiguration of metabolic processes during bud dormancy release. In all, our results reveal the importance of Kac in bud dormancy release and provide a new perspective to understand the molecular mechanisms of seasonal growth of trees.