The IaIA invasion gene of Bartonella bacilliformis encodes a (di)nucleoside polyphosphate hydrolase of the MutT motif family and has homologs in other invasive bacteria
The IaIA invasion gene of Bartonella bacilliformis encodes a (di)nucleoside polyphosphate hydrolase of the MutT motif family and has homologs in other invasive bacteria
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DOI：
10.1006/bbrc.1999.0354
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发表时间：
1999-03-24
影响因子：
3.1
通讯作者：
McLennan, AG
中科院分区：
文献类型：
--
作者：
Cartwright, JL;Britton, P;McLennan, AG
杆状巴尔通体（Bartonella bacilliformis）ialA基因的产物已表达为硫氧还蛋白融合蛋白，它是MutT基序蛋白家族的一种（二）核苷多磷酸水解酶，与植物二腺苷四磷酸水解酶具有很强的序列相似性。它水解具有四个或更多磷酸基团的核苷和二核苷多磷酸，总是产生NTP作为一种产物。二腺苷四磷酸（Ap（4）A）是优选的底物，其Km为10 μ M，k（cat）为3.0 s（-1）。它被Ca ~（2+）和F ~-（Ki = 80 μ M）所抑制。Ap（4）A在（H_2 O）-O-18中水解，得到唯一的标记产物[O-18]AMP。就序列、反应机制和性质而言，IalA与真核生物的Ap（4）A水解酶非常相似，而与先前描述的细菌Ap（4）A水解酶不同。同源物存在于其他侵入性病原体的基因组中。它们的功能可能是：在入侵过程中降低胁迫诱导的二核苷酸水平，从而提高病原体的存活率。(C)北京：科学出版社.
The product of the ialA invasion gene of Bartonella bacilliformis has been expressed as a thioredoxin fusion protein, it is a (di)nucleoside polyphosphate hydrolase of the MutT motif protein family with strong sequence similarity to plant diadenosine tetraphosphate hydrolases. It hydrolyses nucleoside and dinucleoside polyphosphates with four or more phosphate groups, always producing an NTP as one product. Diadenosine tetraphosphate (Ap(4)A) is the preferred substrate with a K-m of 10 mu M and a k(cat) of 3.0 s(-1). It is inhibited by Ca2+ and F- (K-i = 80 mu M). Hydrolysis of Ap(4)A in (H2O)-O-18 yielded [O-18]AMP as the only labelled product. in terms of sequence, reaction mechanism and properties, IalA is very similar to eukaryotic Ap(4)A hydrolases, and unlike previously described bacterial Ap(4)A hydrolases.: Homologs are present in the genomes of other invasive pathogens. They may function: to reduce stress-induced dinucleotide levels during invasion and so enhance pathogen survival. (C) 1999 Academic Press.