BRAIN MICROVESSEL ENDOTHELIAL-CELLS IN TISSUE-CULTURE - A MODEL FOR STUDY OF BLOOD-BRAIN-BARRIER PERMEABILITY
BRAIN MICROVESSEL ENDOTHELIAL-CELLS IN TISSUE-CULTURE - A MODEL FOR STUDY OF BLOOD-BRAIN-BARRIER PERMEABILITY
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DOI：
10.1002/ana.410140403
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发表时间：
1983-01-01
影响因子：
11.2
通讯作者：
GOLDSTEIN, GW
中科院分区：
文献类型：
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作者：
BOWMAN, PD;ENNIS, SR;GOLDSTEIN, GW
从牛脑微血管中制备内皮细胞，并进行组织培养。它们含有VIII因子/血管性血友病抗原，这是可用于确定培养细胞内皮来源的最特异性标记。培养的细胞形成复杂的紧密连接，含有少量的胞饮血管。这些特性是体内血脑屏障形成的原因。当单层内皮细胞短暂暴露于无ca溶液或用1.6马拉比糖处理时，细胞间接触处发生了明显的形态学变化。在这两种情况下，返回控制介质后恢复正常结构。为了评估这些处理对细胞间渗透性的影响，我们测量了14c -蔗糖在胶原涂层尼龙网上培养的内皮细胞单层上的运动。去除外源Ca使蔗糖运动速率提高了120%；阿拉伯糖处理使细胞间通量增加了40%。
Endothelial cells were prepared from bovine brain microvessels and grown in tissue culture. They contained factor VIII/von Willebrand antigen, the most specific marker available for determination of the endothelial origin of cells in culture. The cultured cells formed complex tight junctions and contained few pinocytotic vessels. These properties are responsible for formation of the blood-brain barrier in vivo. When monolayers of the endothelial cells were exposed briefly to a Ca-free solution or treated with 1.6 Marabinose, distinctive morphological changes occurred in the intercellular contacts. In either case, a normal structure was reestablished following return to control medium. To assess the effect of these treatments on transcellular permeability, we measured the movement of 14C-sucrose across a monolayer of endothelial cells cultured on a collagen-coated nylon mesh. Removal of external Ca increased the rate of sucrose movement by 120%; the arabinose treatment increased transcellular flux by 40%.