Changes in the structure of Arabidopsis thaliana during female development of the plant-parasitic nematode Heterodera schachtii
Changes in the structure of Arabidopsis thaliana during female development of the plant-parasitic nematode Heterodera schachtii
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DOI：
10.1007/bf01273172
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发表时间：
1996-01-01
期刊：
影响因子：
2.9
通讯作者：
Sobczak, M
中科院分区：
文献类型：
--
作者：
Golinowski, W;Grundler, FMW;Sobczak, M
甜菜孢囊线虫Heterodera schachtii能够在拟南芥的根和地上部的维管组织中建立摄食结构(合胞)。组织学和超微结构研究被用来评估在单氧条件下幼虫雌性发育过程中的植物反应。在破坏性地入侵根之后，线虫选择并用花柱刺穿单个原形成层细胞，并通过分泌活动将其转变为初始合胞细胞(ISC)。ISC最明显的变化发生在液泡系统和壁上。中央液泡的分化受阻，导致形成许多小液泡。形成多囊泡体和附壁小体。一种电子半透明材料沉积在细胞壁上。细胞壁的部分溶解导致合体的形成。在幼虫最后的成体前发育阶段，合胞体达到最大的纵向和径向伸展，占据中央圆柱体的主要部分。它的特征表明代谢活动水平非常高。肥厚的合胞体被周皮覆盖，其间散布着次生木质部和韧皮部分子。当雌性死亡时，合胞体退化。从根中描述的合胞体的超微结构和组织学特征也在植物的地上部分诱导的合胞体中发现。
The beet cyst nematode Heterodera schachtii is able to establish a feeding structure (syncytium) in the vascular tissue of roots and shoots of Arabidopsis thaliana. Histological and ultrastructural studies were performed to assess plant responses during the development of juvenile females under monoxenic conditions. After destructively invading a root the nematode selects and pierces a single procambial cell with its styler and transforms it into an initial syncytial cell (ISC) by secretory activity. The first most obvious changes in the ISC occur in the vacuolar system and at the wall. Differentiation of a central vacuole is impeded resulting in the formation of numerous small vacuoles. Multivesicular and paramural bodies are formed. An electron translucent material is deposited on the cell wall. Partial dissolution of the cell wall leads to the formation of a syncytium. At the juveniles' last pre-adult developmental stage the syncytium attains its maximum longitudinal and radial extension, occupying a major part of the central cylinder. Its features are indicative of a very high level of metabolic activity. The hypertrophied syncytium is ensheathed by a peridermal cover in which secondary xylem and phloem elements are interspersed. When females die the syncytia degenerate. The ultrastructural and histological features of syncytia described from roots are also found in syncytia induced in aerial parts of the plant.