Natural therapeutic agents for neurodegenerative diseases from the shells of Xanthoceras sorbifolium
Natural therapeutic agents for neurodegenerative diseases from the shells of Xanthoceras sorbifolium
复制标题
文冠果壳中的神经退行性疾病天然治疗剂
DOI：
10.1016/j.bioorg.2020.104038
复制
发表时间：
2020-08-01
影响因子：
5.1
通讯作者：
Li, Ning
中科院分区：
文献类型：
--
作者：
Chen, Gang;Xie, Yumeng;Li, Ning
神经炎症与神经退行性疾病有关，表现为小胶质细胞释放的一氧化氮（NO）过度产生。然而，到目前为止，还没有有效的治疗或预防神经退行性疾病的策略。文冠果中的三萜皂苷被证明能够在神经退行性疾病中引起保护作用。因此，本文对黄藤70%乙醇提取物进行了系统的化学研究。从山梨叶中鉴定出22种化合物，包括4种以前未描述的三萜皂苷和14种已知的三萜皂苷，以及沿着4种生物碱。通过理化和光谱方法鉴定了它们的结构。用基于场的3D-QSAR模型预测1-18的体内抗AD作用，并通过评估LPS诱导的NO产生和检查iNOS、TNF-α、IL-1 β和IL-6的水平来测定BV-2细胞中的抗神经炎活性以支持预测的结果。因此，化合物14、16、19和20由于其有效的抗神经炎症活性而具有治疗神经退行性疾病的潜力。
Neuroinflammation is linked to neurodegenerative diseases, manifested by the microglial-released over -pro-duction of nitric oxide (NO). However, so far there is no effective strategy regarding curing or preventing neurodegenerative diseases. Triterpene saponins from Xanthoceras sorbifolium were proved to be capable of eliciting a protective effect in neurodegenerative diseases. Thus, a systematic chemical study on the 70% ethanol extract of X. sorbifolium was conducted, leading to the identification of 22 compounds, including four previously undescribed triterpenes saponins and 14 known ones, along with four alkaloids. Their structures were elucidated by physicochemical and spectral methods. The in vivo anti-AD effects of 1-18 were predicted with a field-based 3D-QSAR model and anti-neuroinflammatory activities were assayed in BV-2 cells by assessing LPS-induced NO production and examine levels of iNOS, TNF-alpha, IL-1 beta, and IL-6 to support the predicted results. As a result, compounds 14, 16, 19, and 20 could have therapeutic potentials for neurodegenerative diseases due to their potent anti-neuroinflammatory activities.