Expression artifact with retroviral vectors based on pBMN.
Expression artifact with retroviral vectors based on pBMN.
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基于 pBMN 的逆转录病毒载体的表达产物。
DOI：
10.1016/j.ab.2009.07.014
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发表时间：
2009
影响因子：
2.9
通讯作者：
Wheelock,MargaretJ
中科院分区：
文献类型：
--
作者：
Fukunaga,Yoshitaka;Svoboda,RobertA;Cerny,RonaldL;Johnson,KeithR;Wheelock,MargaretJ
在表征p120连环蛋白的各种剪接形式时，我们观察到p120似乎是一种新的翻译后修饰，导致依赖于剪接模式的更高分子量形式。进一步的研究表明，较高分子量的形式是由逆转录病毒载体编码的序列与p120之间的融合蛋白。我们发现我们使用的公开可用的向量序列与实验序列不一致。我们提醒其他调查人员注意这个潜在的人工制品。
While characterizing various splice forms of p120 catenin, we observed what appeared to be a novel posttranslational modification of p120, resulting in a higher molecular weight form that was dependent on the splicing pattern. Further investigation revealed the higher molecular weight form to be a fusion protein between sequences encoded by the retroviral vector and p120. We found that the publicly available sequence of the vector we used does not agree with the experimental sequence. We caution other investigators to be aware of this potential artifact.