Endothelial precursors and mature endothelial cells are increased in the peripheral blood of myelodysplastic syndromes
Endothelial precursors and mature endothelial cells are increased in the peripheral blood of myelodysplastic syndromes
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DOI：
10.1080/10428190500144235
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发表时间：
2005-09-01
影响因子：
2.6
通讯作者：
Deliliers, GL
中科院分区：
文献类型：
--
作者：
Cortelezzi, A;Fracchiolla, NS;Deliliers, GL
血管生成增加已被证明是许多实体瘤的一个重要预后因素。在肿瘤血液学环境中，它与骨髓增生异常综合征(MDS)、慢性髓系白血病、急性淋巴细胞性白血病和髓系白血病有关。近年来，循环内皮细胞(CECs)增多与乳腺癌和非霍奇金淋巴瘤(NHL)有关。基于这些前提，我们分析了50名MDS患者和20名健康献血者的总CEC和激活的CEC，以及内皮前体(CEP)。用流式细胞仪对CECs和CEP进行定量。CEC水平与骨髓微血管密度(MVD)进行比较。此外，还检测了25例MDS患者血清中血管内皮生长因子(VEGF)、碱性成纤维细胞生长因子(BFGF)和可溶性血管内皮生长因子受体2(VEGFR2)水平。与对照组相比，MDS患者总的、激活的CECs和CEP显著增加(p<；0.0001)；而在MDS患者中，未发现与法裔美国人英国人(FAB)、国际预后评分系统(IPSS)亚型或生存期相关。CEC越高的患者MVD也越高。在所分析的细胞因子中，sVEGFR2在较低的IPSS风险级别中显著升高，而bFGF水平与总的和激活的CEC直接相关。综上所述，这些数据强化了血管生成在MDS发病机制中可能起作用的假设。
Increased angiogenesis has been demonstrated to be a significant prognostic factor in many solid tumors. In the oncohematological setting, it has been associated with myelodysplastic syndromes (MDS), chronic myeloid leukemia, acute lymphoid, and myeloid leukemias. Recently, increased circulating endothelial cells (CECs) have been associated with breast cancer and non-Hodgkin lymphoma (NHL). Based on these premises we analysed total and activated CECs, and endothelial precursors (CEPs) in 50 MDS patients and 20 healthy donors. CECs and CEPs were quantified by flow cytometry. CEC levels were compared with bone marrow ( BM) microvessel density (MVD). In addition, some angiogenic factors, namely vascular endothelial growth factor (VEGF), basic fibroblast growth factor ( bFGF) and soluble VEGF-Receptor2 (VEGFR2), were tested in the sera from 25 MDS patients. Total, activated CECs and CEPs were significantly increased in MDS when compared to control group (p < 0.0001); whereas in the MDS cases no association was found with French-American British (FAB), International Prognostic Scoring System (IPSS) subtypes or survival. Patients with higher CECs also showed higher MVD. Among the cytokines analysed, sVEGFR2 was significantly higher in the lower IPSS risk classes, while the levels of bFGF directly correlated with total and activated CECs. Taken together these data strengthen the hypothesis of a possible role of angiogenesis in MDS pathogenesis.