A bird's eye view tracking slow nanometer-scale movements of single molecular nano-assemblies.
A bird's eye view tracking slow nanometer-scale movements of single molecular nano-assemblies.
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DOI：
10.1016/s0076-6879(10)75006-0
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发表时间：
2010
影响因子：
--
通讯作者：
Walter, Nils G.
中科院分区：
文献类型：
--
作者：
Michelotti, Nicole;de Silva, Chamaree;Johnson-Buck, Alexander E.;Manzo, Anthony J.;Walter, Nils G.
单粒子荧光追踪方法的最新改进使得可以对纳米尺度上的分子运动进行详细研究。为了将这些工具引入新兴的 DNA 纳米技术领域，我们利用一纳米精度的荧光成像 (FIONA) 和单分子高分辨率共定位 (SHREC) 来监测合成分子行走器（称为“蜘蛛”）在单分子水平上的扩散行为。在这里，我们讨论所使用的成像方法、在伪一维表面上跟踪单个蜘蛛的结果，以及这些纳米步行者的低速度（~3 nm/min）带来的一些独特的实验挑战。这些实验证明了荧光粒子追踪作为在单分子水平上详细表征合成分子纳米系统的工具的前景。
Recent improvements in methods of single-particle fluorescence tracking have permitted detailed studies of molecular motion on the nanometer scale. In a quest to introduce these tools to the burgeoning field of DNA nanotechnology, we have exploited fluorescence imaging with one-nanometer accuracy (FIONA) and single-molecule high-resolution colocalization (SHREC) to monitor the diffusive behavior of synthetic molecular walkers, dubbed “spiders”, at the single-molecule level. Here we discuss the imaging methods used, results from tracking individual spiders on pseudo-one-dimensional surfaces, and some of the unique experimental challenges presented by the low velocities (~3 nm/min) of these nanowalkers. These experiments demonstrate the promise of fluorescent particle tracking as a tool for the detailed characterization of synthetic molecular nanosystems at the single-molecule level.