Selenium Alleviates Inflammation in Staphylococcus aureus-Induced Mastitis via MerTK-Dependent Activation of the PI3K/Akt/mTOR Pathway in Mice
Selenium Alleviates Inflammation in Staphylococcus aureus-Induced Mastitis via MerTK-Dependent Activation of the PI3K/Akt/mTOR Pathway in Mice
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DOI：
10.1007/s12011-021-02794-z
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发表时间：
2021-06-29
影响因子：
3.9
通讯作者：
Hu, Chang-min
中科院分区：
文献类型：
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作者：
Chen, Si-jie;Zhang, Chen-yuan;Hu, Chang-min
金黄色葡萄球菌感染引起的乳腺炎不仅造成严重的经济损失，而且影响人类健康。硒在机体免疫中具有重要作用。硒对乳杆菌性乳腺炎的调控机制尚不清楚。金黄色葡萄球菌仍然主要未知。本研究旨在探讨硒对乳杆菌性乳腺炎的抑制作用。金黄色葡萄球菌通过调节MerTK。将60只雌性BALB/c小鼠随机分为低硒对照组（LSN）、正常硒对照组（NSN）、高硒对照组（HSN）、低硒对照组（SN）、正常硒对照组（NSN）。金黄色葡萄球菌组（LSS）、硒正常的S.金黄色葡萄球菌组（NSS）、高硒S.金黄色葡萄球菌组（HSS）。通过组织病理学染色、western blot分析、酶联免疫吸附试验和qRT-PCR检测Se对MerTK的调节作用。随着硒浓度的增加，IL-1 β、IL-6和TNF-α的水平降低，而MerTK、PI 3 K、AKT和mTOR的磷酸化水平升高。因此，本研究表明，硒可以缓解S。金黄色葡萄球菌乳腺炎的发生与MerTK和PI 3 K/AKT/mTOR通路的激活有关。
Mastitis caused by Staphylococcus aureus infection not only causes serious economic losses, but also affects human health. Se plays an important role in body immunity. However, the mechanisms by which Se regulates mastitis induced by S. aureus are still principally unknown. The purpose of this study is to investigate whether Se can inhibit mastitis induced by S. aureus through regulation of MerTK. Sixty BALB/c female mice were fed low, normal, or high Se concentrations for 7 weeks and then randomly divided into six groups (Se-Low Control group (LSN), Se-Normal Control group (NSN), Se-High Control group (HSN), Se-Low S. aureus group (LSS), Se-Normal S. aureus group (NSS), Se-High S. aureus group (HSS)). The regulation of Se on MerTK was detected via histopathological staining, western blot analysis, enzyme-linked immunosorbent assay, and qRT-PCR. With increased selenium concentrations, the levels of IL-1 beta, IL-6, and TNF-alpha decreased, while the phosphorylation levels of MerTK, PI3K, AKT, and mTOR increased. Therefore, this study showed that Se could alleviate S. aureus mastitis by activating MerTK and PI3K/AKT/mTOR pathway.