Biochemical survey of the polar head of plant glycosylinositolphosphoceramides unravels broad diversity
Biochemical survey of the polar head of plant glycosylinositolphosphoceramides unravels broad diversity
复制标题
DOI：
10.1016/j.phytochem.2013.08.002
复制
发表时间：
2013-12-01
期刊：
影响因子：
3.8
通讯作者：
Mongrand, Sebastien
中科院分区：
文献类型：
--
作者：
Cacas, Jean-Luc;Bure, Corinne;Mongrand, Sebastien
尽管糖基肌醇磷酸神经酰胺(GIPC)是植物组织中的主要鞘磷脂，但它们的结构特征仍然很差。这种缺乏信息，特别是关于极地头部的信息，目前阻碍了对生物系统中GIPC功能的理解。这种情况促使我们对植物GIPC进行了大规模的分析：在不同的系统发育类群中选择了23种植物，调查了它们的GIPC总含量。提取GIPC，并用负离子MALDI和ESI质谱仪对其极头进行表征。我们的数据揭示了植物中GIPC分布的出人意料的广泛多样性，以及在绿藻和红藻中出现尚未报道的GIPC结构。在单子叶植物中，含有三种糖的GIPC显然是主要的，而在一些值得注意的例外情况下，含有两种糖的系列在真二叶植物中占主导地位。在植物细胞培养中，GIPC极头似乎比它们起源的组织中含有更多数量的多糖单位。从GIPC的代谢、多样性和功能方面对其前景进行了讨论。(C)2013爱思唯尔有限公司。保留所有权利。
Although Glycosyl-Inositol-Phospho-Ceramides (GIPCs) are the main sphingolipids of plant tissues, they remain poorly characterized in term of structures. This lack of information, notably with regard to polar heads, currently hampers the understanding of GIPC functions in biological systems. This situation prompted us to undertake a large scale-analysis of plant GIPCs: 23 plant species chosen in various phylogenetic groups were surveyed for their total GIPC content. GIPCs were extracted and their polar heads Were characterized by negative ion MALDI and ESI mass spectrometry. Our data shed light on an unexpected broad diversity of GIPC distributions within Plantae, and the occurrence of yet-unreported GIPC structures in green and red algae. In monocots, GIPCs with three saccharides were apparently found to be major, whereas a series with two saccharides was dominant in Eudicots within a few notable exceptions. In plant cell cultures, GIPC polar heads appeared to bear a higher number of glycan units than in the tissue from which they originate. Perspectives are discussed in term of GIPC metabolism diversity and function of these lipids. (C) 2013 Elsevier Ltd. All rights reserved.